Importance of the lumpy skin disease virus (LSDV) LSDV126 gene in differential diagnosis and epidemiology and its possible involvement in attenuation.

Examination of lumpy skin disease virus (LSDV) isolates from different geographic regions and times revealed that assays developed in our laboratory for differentiating between virulent Israeli viruses and Neethling vaccine virus (NVV) are generally useful in most, if not all, endemic areas in which NVV-based vaccines are used. Recently it was revealed that the LSDV126 gene of field isolates contains a duplicated region of 27 bp (9 aa), while the vaccine viruses have only one copy. Phylogenetic analysis of a 532-bp segment carrying the LSDV126 gene and whole virus genome sequences revealed that LSDV isolates formed two groups: virulent and vaccine viruses.

Multiple invasions of O1 FMDV serotype into Israel revealed by genetic analysis of VP1 genes of Israeli's isolates from 1989 to 2007.

Foot-and-mouth disease virus (FMDV), one of the most dangerous viruses affecting cloven-hoofed animals, comprises seven serotypes that do not mutually cross-protect, with a total of about 80 subtypes. The Middle East is an FMD-endemic region, with repeated FMD outbreaks and In spite of its compulsory vaccination policy in Israel, outbreaks occur repeatedly. In order to compare the Israeli isolates, the complete viral VP1 genes of representative viruses isolated during the major outbreaks from 1989 to 2007 were sequenced and subjected to phylogenetic analysis, which showed that each outbreak was initiated by introduction of a new virus lineage and not by endemic and resident viruses.