Collagen production in cardiac fibroblasts during inhibition of aminopeptidase B.

Objective: To determine whether the aminopeptidase B inhibitor, arphamenine A, could affect collagen production and expression in control and TGF-ss1-treated cardiac fibroblasts.

Design And Methods: Cardiac fibroblasts from passage 2 from normal male adult rats were cultured to confluency and incubated with and without 600 pmol/l TGF-ss1 for 2 days in serum-free Dulbecco's modified Eagle's medium and then incubated with 100 mol/l arphamenine A for 1 day in this medium with added ascorbic acid, ss-aminopropionitrile and titriated proline. Soluble collagen was measured in the conditioned medium and non-soluble collagen in the cell layer.

Pediatric residency training on domestic violence: a national survey.

Objective: Between 3.3 and 10 million children witness domestic violence (DV) each year. These children are at risk for both emotional and behavioral problems.