Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection.

Background: Molecular DNA cloning is crucial to many experiments and with the trend to higher throughput of modern approaches automated techniques are urgently required. We have established an automated, fast and flexible low-cost expression cloning approach requiring only vector and insert amplification by PCR and co-transformation of the products.

Results: Our vectors apply positive selection for the insert or negative selection against empty vector molecules and drive strong expression of target proteins in E.