The Diversity of Serum Anti-DSG3 IgG Subclasses Has a Major Impact on Pemphigus Activity and Is Predictive of Relapses After Treatment With Rituximab.

Introduction: We studied the distribution and pathogenicity of anti-DSG3 IgG subclasses during the course of pemphigus vulgaris (PV).

Methods: We longitudinally studied the distribution of anti-DSG3 IgG subclasses (before after treatment) in sera from PV patients, using an addressable-laser bead immunoassay (ALBIA). The pathogenicity of corresponding sera was tested using keratinocyte dissociation and immunofluorescence assays.

Longitudinal Pathogenic Properties and -Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.

Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins. Systemic corticosteroids usually decrease the titers of anti-desmoglein autoantibodies and improve patients' conditions. Since modifications of IgG -glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their -glycosylation profile.