Publications by authors named "Mario Gamboa"
Article Synopsis
- Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are growing global health issues linked to a genetic risk factor (rs738409) that disrupts lipid processing in the liver, leading to complications such as liver fibrosis.* -
- Research has identified the drug momelotinib, originally developed for myelofibrosis, as a potent down-regulator of this harmful genetic expression, showing significant reductions in liver cell mRNA levels in lab tests and animal models.* -
- The drug's effectiveness is linked to changes in gene expression regulation, possibly altering chromatin accessibility, and suggests it could be a promising treatment option for various stages of NA
Epithelial organoids derived from human donor tissues are important tools in fields ranging from regenerative medicine to drug discovery. Organoid culture requires expansion of stem/progenitor cells in Matrigel, a tumor-derived extracellular matrix (ECM). An alternative completely synthetic ECM could improve reproducibility, clarify mechanistic phenomena, and enable human implantation of organoids.
View Article and Find Full Text PDFMicrophysiological systems (MPSs) are in vitro models that capture facets of in vivo organ function through use of specialized culture microenvironments, including 3D matrices and microperfusion. Here, we report an approach to co-culture multiple different MPSs linked together physiologically on re-useable, open-system microfluidic platforms that are compatible with the quantitative study of a range of compounds, including lipophilic drugs. We describe three different platform designs - "4-way", "7-way", and "10-way" - each accommodating a mixing chamber and up to 4, 7, or 10 MPSs.
View Article and Find Full Text PDF