Catechin versus MoS Nanoflakes Functionalized with Catechin: Improving the Sperm Fertilizing Ability-An In Vitro Study in a Swine Model.

Nowadays, the adoption of In Vitro Fertilization (IVF) techniques is undergoing an impressive increase. In light of this, one of the most promising strategies is the novel use of non-physiological materials and naturally derived compounds for advanced sperm preparation methods. Here, sperm cells were exposed during capacitation to MoS/Catechin nanoflakes and catechin (CT), a flavonoid with antioxidant properties, at concentrations of 10, 1, 0.

The impact of five years storage/biobanking at -80°C on mouse spermatozoa fertility, physiology, and function.

Background: We previously demonstrated how mouse spermatozoa can be efficiently stored for two years in a -80°C freezer, maintaining their ability to fertilize mouse eggs.

Objectives: The main objective here was to evaluate the effects of five years at -80°C compared to liquid nitrogen storage (LN , control condition) on mouse sperm viability, physiological parameters, and fertilization capacity.

Materials And Methods: Three different strains were used: C57BL/6N, C57BL/6J and CD1.

Shape based kinetic outlier detection in real-time PCR.

Background: Real-time PCR has recently become the technique of choice for absolute and relative nucleic acid quantification. The gold standard quantification method in real-time PCR assumes that the compared samples have similar PCR efficiency. However, many factors present in biological samples affect PCR kinetic, confounding quantification analysis.

Cancer mortality among men occupationally exposed to dichlorodiphenyltrichloroethane.

Several studies have evaluated cancer risk associated with occupational and environmental exposure to dichlorodiphenyltrichloroethane (DDT). Results are mixed. To further inquire into human carcinogenicity of DDT, we conducted a mortality follow-up study of 4,552 male workers, exposed to DDT during antimalarial operations in Sardinia, Italy, conducted in 1946 to 1950.