Enantiomeric fractionation as a tool for quantitative assessment of biodegradation: The case of metoprolol.

An efficient chiral liquid chromatography high resolution mass spectrometry method has been developed for the determination of metoprolol (MTP) and three of its major metabolites, namely O-desmethylmetoprolol (O-DMTP), α-hydroxymetoprolol (α-HMTP) and metoprolol acid (MTPA) in wastewater treatment plant (WWTP) influents and effluents. The optimized analytical method has been validated with good quality parameters including resolution >1.3 and method quantification limits down to the ng/L range except for MTPA.

Insights into reductive dechlorination of triclocarban in river sediments: Field measurements and in vitro mechanism investigations.

Triclocarban (TCC) reductive dechlorination was investigated using a combination of field and laboratory experiments. Field monitoring revealed that TCC reductive dechlorination in river sediments leads to formation of two isomers of its lesser chlorinated congener namely 3,4'-dichlorocarbanilide and 4,4'-dichlorocarbanilide. Monochlorocarbanilide was not detected in sediments supporting that transformation of dichlorocarbanilide into monochlorocarbanilide is the rate limiting step of TCC dechlorination.

Screening triclocarban and its transformation products in river sediment using liquid chromatography and high resolution mass spectrometry.

An analytical method was developed and validated for the target screening of triclosan (TCS), triclocarban (TCC) and its lesser and higher chlorinated congeners namely, 4,4'-dichlorocarbanilide (DCC) 3,3',4,4'-tetrachlorocarbanilide (3-Cl-TCC) and 2,3',4,4'-tetrachlorocarbanilide (2-Cl-TCC) in river sediment. Sediment samples were extracted by pressurized liquid extraction and quantification and identification of target compounds were carried by liquid chromatography high resolution mass spectrometry (LC-HRMS). The overall method recoveries were 89% with relative standard deviations below 6%.