Perception of the value of simulation models by dental anesthesia teachers and implementation of simulation models for teaching.

Background: A dental anesthesia simulation model (DASM) is a tool for the teaching and learning of local dental anesthesia techniques. We investigated how dental anesthesia teachers (DATs) perceive the value of those tools.

Objective: The objective of this study was to investigate the following: the characteristics of the dental anesthesia curriculum used by DATs in Mexico; the DATs' opinion of ethical issues in the teaching of local dental anesthesia; the DATs' perception of the value of using a DASM to teach local dental anesthesia; and the characteristics of the implementation of a DASM by DATs.

Microtomographic and histological evaluation of two bioceramics as pulp capping agents in vivo.

Maintaining pulp vitality and function is a priority of the medicaments employed in pulp therapy to preserve tooth integrity. Aim: This study evaluated inflammatory response and reparative dentin bridge formation after direct pulp capping with two different bioceramics. Materials and Method: This was an in vivo controlled experimental study on 12 male Wistar rats.

Efficacy of alendronic acid solution in removal calcium hydroxide from root canals.

Background: Intracanal medication is considered an alternative after instrumentation in the case of pulp necrosis. However, the elimination of this medication plays an important role in the obturation process, which is why various chelating solutions have currently been studied for this purpose. Therefore, this study aimed to analyze the irrigant based on alendronic acid (AA), which contains in its chemical structure functional groups capable of forming complexes with Ca2+ ions.

Effect of polyethylene fiber orientation on fracture resistance of endodontically treated premolars.

Statement Of Problem: Polyethylene fibers have been reported to improve the fracture resistance of endodontically treated teeth, but their optimal orientation is unclear.

Purpose: The purpose of this in vitro study was to evaluate the effects of different fiber positions and orientations on the fracture resistance of endodontically treated premolar teeth.

Material And Methods: One hundred endodontically treated maxillary premolars were divided into 2 groups according to the cavity design, mesio-occluso-distal and occluso-buccal, and each group was divided into 5 subgroups (n=10) according to the polyethylene fiber orientation on the pulpal floor: unidirectional horizontal (from mesial to distal), unidirectional vertical (from buccal to palatal), bidirectional (buccopalatal and mesiodistal), circular (around the walls), and without fibers (control group).

Effect of degree of conversion on the surface properties of polymerized resin cements used for luting glass fiber posts.

Statement Of Problem: Lack of conversion of resin cements for luting glass fiber posts in deeper levels of the root canal may compromise clinical performance.

Purpose: The purpose of this in vitro study was to evaluate the effect of the degree of conversion on the surface properties of dual-polymerized resin cements used for cemented glass fiber posts according to the root level.

Material And Methods: Fifty maxillary central incisor teeth were endodontically treated, and glass fiber posts were cemented using the following systems: self-adhesive dual-polymerized resin luting cement (RelyX U200) and dual-polymerizing flowable core and a post luting system (Rebilda DC) with a self-etching adhesive (Futurabond DC).

Effect of short fiber-reinforced composite combined with polyethylene fibers on fracture resistance of endodontically treated premolars.

Statement Of Problem: Whether direct coronal restorations of endodontically treated teeth with short fiber-reinforced composite combined with polyethylene fibers provide adequate mechanical strength is unclear.

Purpose: The purpose of this in vitro study was to compare the fracture strength of endodontically treated premolars with standardized mesio-occluso-distal (MOD) preparations restored with short fiber-reinforced composite (SFRC) combined with polyethylene Ribbond fibers (PRFs).

Material And Methods: A total of 40 premolars were selected and distributed into 4 groups (n=10) as follows: group restored with SFRC (EverX Posterior), group restored with PRF (Ribbond fibers), and group restored with the combination PRF+SFRC, all followed by a conventional composite resin (IPS Empress Direct).

Antibiofilm properties of silver nanoparticles incorporated into polymethyl methacrylate used for dental applications.

Background: Acrylic resins used in dental and biomedical applications do not have antimicrobial properties, their surface is susceptible to colonization of microorganisms.

Objective: The aim of this study was to evaluate the antibiofilm properties of silver nanoparticles (AgNPs) deposited in a polymethyl methacrylate (PMMA) surface against a Staphylococcus aureus biofilm.

Methods: The PMMA was impregnated with AgNPs by using the in-situ polymerization method.

Effect of tricalcium silicate cements in gene expression of , , and , and cell adhesion in primary teeth' pulp fibroblasts.

Tricalcium silicate cements (TSCs) regulate gene expression and cell responses from dental tissues surrounding the repair site. The study aimed to evaluate the gene expression levels of Collagen Type I Alpha 1 Chain (COL1A1), Mitogen-Activated Protein Kinases (MAPK's), Nuclear Factor-Kappa B (NF-κB), cell adhesion, and morphology of human dental pulp fibroblasts (hDPFs) from primary teeth treated with eluates obtained from Mineral Trioxide Aggregate (MTA) and Biodentine. hDPFs were treated with eluates from Biodentine and MTA (2.

Effect of microwave polymerization on the thermomechanical and surface properties of ocular prosthetic acrylic resins.

Background: Polymerization conditions affect the physical-mechanical properties of acrylic resins used for craniofacial prostheses.

Objective: The aim of this study was to evaluate the effect of microwave polymerization on the thermomechanical properties and surface morphology of ocular prostheses fabricated with polymethyl methacrylate (PMMA).

Methods: PMMA discs were polymerized with microwave energy and with conventional heat polymerization (CHP) method.

Surface degradation and biofilm formation on hybrid and nanohybrid composites after immersion in different liquids.

Purpose: This study evaluated the association of surface degradation and formation of Streptococcus mutans (S. mutans) biofilm in resin-based composites (RBCs) after storage in different acidic liquids.

Methods: To evaluate microhardness and surface micromorphology, hybrid and nanohybrid RBC discs were stored in artificial gastric acid, cola drink, orange juice, artificial saliva, and distilled water for three intervals of 15 min per day for 7, 15, and 30 days.

3D-Printed Tubular Scaffolds Decorated with Air-Jet-Spun Fibers for Bone Tissue Applications.

The fabrication of instructive materials to engineer bone substitute scaffolds is still a relevant challenge. Current advances in additive manufacturing techniques make possible the fabrication of 3D scaffolds with even more controlled architecture at micro- and submicrometric levels, satisfying the relevant biological and mechanical requirements for tissue engineering. In this view, integrated use of additive manufacturing techniques is proposed, by combining 3D printing and air-jet spinning techniques, to optimize the fabrication of PLA tubes with nanostructured fibrous coatings for long bone defects.

Effects of drug abuse and mental disorders on oral health: A case report.

Substance use disorders, related to mental health issues can lead to oral pathologies. The purpose of this case report was to identify the oral conditions and manifestations in a 28-year-old male narco-dependent schizophrenic who smoked tobacco and marijuana. In intra-oral clinical exploration, poor oral hygiene, excessive tartar formation and dental plaque were observed, and the diagnosis of severe chronic periodontitis was established.

Effect of 4-Allyl-1-hydroxy-2-methoxybenzene (eugenol) in the expression of genes involved in cellular cycle and apoptotic process in dental pulp fibroblasts.

Objective: This study sought to evaluate the effect of eugenol on the cell morphology and expression of genes involved in the apoptotic process in human dental pulp fibroblasts (hDPFs) from deciduous teeth.

Materials And Methods: hDPFs were cultured with 4 concentrations of eugenol (0.06 nM, 0.

Evaluation of the sealing capability and morphological fit of prefabricated dowels and fiber-reinforced composite resin: An in vitro study.

Statement Of Problem: Prefabricated dowels do not always provide intraradicular sealing in the root canal dentin, and the lack of sealing predisposes the dowel to adhesive failure and debonding. It is unclear if fiber-reinforced composite resin dowels provide better sealing.

Purpose: The purpose of this in vitro study was to evaluate the intraradicular sealing and morphological fit of prefabricated dowels and fiber-reinforced composite resin dowels in root canal dentin.

Frequency of Pathogenic Microorganisms in Removable Orthodontic Appliances and Oral Mucosa in Children.

Objective: The aim of this study was to evaluate the frequency of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida species in removable orthodontic appliances (ROA) and the support oral mucosa in children.

Study Design: The study participants comprised 55 patients aged 6-12 years requiring ROA. The samples of biofilm colonization from the support oral mucosa and the ROA were taken prior to the use of the ROA (T0) and 4 weeks (T1) after ROA placement.

Morphological and biological properties of silica nanoparticles for CRTC3-siRNA delivery and downregulation of the RGS2 expression in preadipocytes.

The aim of this study was to characterize the morphological properties of amorphous silica nanoparticles (SiO NPs), their cytotoxicity and intracellular location within Human Osteoblasts (HOB). Additionally, SiO NPs were explored for their effectivity as carriers of CRTC3-siRNA on Human Preadipocytes (HPAd), and thus downregulate RGS2 gene expression. SiO NPs were synthesized using the method of Stöber at 45 °C, 56 °C, and 62 °C.

Reinsertion of a fractured clinical crown as a biological restoration after dental trauma.

This clinical report describes the rehabilitation procedure of an upper central tooth with a coronal fracture without compromising the root. The treatment plan consisted of the reinsertion of the fractured fragment as a definitive cemented biological restoration. Due to the deficient remnant, a fiber-reinforced composite post was used.

Does microwave and hydrogen peroxide disinfection reduce Candida albicans biofilm on polymethyl methacrylate denture surfaces?

Statement Of Problem: Whether the disinfection of polymethyl methacrylate (PMMA) dentures eliminates Candida albicans biofilm is unclear.

Purpose: The purpose of this in vitro study was to determine the antimicrobial effect of immersion in hydrogen peroxide (HO) and subsequent application of microwaves on the formation of C albicans biofilm on the surface of polished and unpolished PMMA disks.

Material And Methods: Polished and unpolished PMMA disks (n=40) were mounted in a Center for Disease Control (CDC) biofilm reactor by adding yeast-dextrose-peptone (YPD) broth inoculated with C albicans in a cell suspension for 24 hours.

Antimicrobial effect of gold nanoparticles in the formation of the Staphylococcus aureus biofilm on a polyethylene surface.

The main of this study was to evaluate the inhibitory effect on the in vitro formation of the Staphylococcus aureus biofilm formed on a polyethylene (PE) surface with a nanostructured Gold (Au) coating for medical devices. An experimental in vitro study was carried out using PE discs with an Au nanoparticle coating (AuNPs) on one side (experimental group) and without coating on the other (control group); the discs were mounted in the CDC biofilm reactor adding broth of yeast-dextrose-peptone (YPD) sterile culture inoculated with S. aureus in a cell suspension (5 × 10 cells/ml).

Effect of Five Commercial Probiotic Formulations on Candida Albicans Growth: In Vitro Study.

Purpose: The objective was to evaluate the antagonistic effect of Lactobacillus and Bifidobacterium recovered from five commercial probiotics on the growth of C. albicans.

Study Design: The Lactobacillus and Bifidobacterium strains of five commercial probiotics were recovered and grown: Probio Hp+®, ProBiseis®, Lactipan®, Liolactil®, and Lacteol Fort®; 50 mg of each was hydrated and grown in Lactobacilli MRS (De Man, Rogosa and Sharpe) broth and incubated at 37°C with stirring (120 RPM) for 24 hours.

Evaluation of the Bond Strength and Marginal Seal of Indirect Restorations of Composites Bonded with Preheating Resin.

Objective:  Preheated resins (PR) are considered a cementing agent option for indirect adhesive restorations of composite inlays and onlays. The objective of this in vitro study was to evaluate the marginal sealing, adhesive interface, and microtensile bond strength of indirect adhesive restorations of composites in terms of dentin cemented with PR.

Materials And Methods:  Standardized Class II preparations were performed on 30 extracted human premolars, impressions were taken, and indirect composite restorations were manufactured.

Effect of dental restorative materials surface roughness on the in vitro biofilm formation of Streptococcus mutans biofilm.

Purpose: To evaluate Streptococcus mutans biofilm formation over different restorative dental materials.

Methods: Using a bioreactor over 72 hours, four commercially available ceramics were evaluated: IPS E-max Press, IPS E-max CAD, Lava Ultimate CAD-CAM, Vita Enamic and two resin composites (SR Nexco Paste and Brilliant NG). The results were evaluated using atomic force microscopy and confocal microscopy, the biofilm was stained and the arbitrary fluorescence units (AFU) quantified.

Postextraction Alveolar Preservation and Use of the Crown of the Extracted Tooth as a Temporary Restoration.

Ranging from the extraction of teeth from anterior sector commitment to the loss of bone and soft tissues, alveolar preservation is a regenerative technique that employs biomaterials that induce the preservation of bone tissue and minimize resorption. Placement of provisional restorations at the defect site maintains the stability of the soft tissues and aesthetics at which the definitive restoration is found. The objective of this clinical case was alveolar preservation postextraction of the anterior tooth that presented a coronal fracture, placing the clinical crown of the extracted tooth as the provisional restoration to maintain stability and to avoid soft tissue collapse and compromise to an aesthetic area.