New parameters of diploid histogram of image DNA cytometry and newly characterized types of nucleolar organizer region structures in defining the proliferative-kinetic index in chronic leukemic lymphoproliferative disorders.

Objective: To introduce new parameters of diploid histogram of image DNA cytometry and new types of silver-stained nucleolar organizer regions (AgNORs) and to validate resulting proliferative-kinetic index (PKI) in a prognostic study of patients with chronic leukemic lymphoproliferative disorders (CLLPD).

Study Design: A total of 413 smears of from various tumor mass compartments-bone marrow, peripheral blood and lymph node-were analyzed in CLLPD as a whole, as well as separately in the B-chronic lymphocytic leukemia with variants (B-CLL+V). The analysis of the diploid histogram included percentage of cells at the peak of the DNA histogram and percentage of cells with lower and higher contents of DNA than cells at the peak.

[Clinical and laboratory prognostic parameters for leukemic types of chronic lymphoproliferative diseases].

Aim: The aim of the study was to identify the clinical and laboratory (hematologic, biochemical and morphological) prognostic parameters of chronic leukemic lymphoproliferative diseases (CLLPD).

Methods: The study included 155 CLLPD patients. Analysis was performed in the overall CLLPD population and separately in a subgroup of patients with B chronic lymphocytic leukemia with variants (B-CLL+V) including typical B chronic lymphocytic leukemia (B-CLL), mixed chronic lymphocytic leukemia and prolymphocytic leukemia (CLL/PLL), and a variant of chronic lymphocytic leukemia with lymphoplasmocytoid differentiation (CLL/IMC).