Factors affecting mixed-mode retention properties of cation-exchange stationary phases.

Cation-exchange stationary phases were characterized in different chromatographic modes (HILIC, RPLC, IC) and applied to the separation of non-charged hydrophobic and hydrophilic analytes. The set of columns under investigation included both commercially available cation-exchangers and self-prepared PS/DVB-based columns, the latter consisting of adjustable amounts of carboxylic and sulfonic acid functional groups. The influence of cation-exchange site and polymer substrate on the multimodal properties of cation-exchangers was identified using selectivity parameters, polymer imaging and excess adsorption isotherms.

Mixed-acidic cation-exchange material for the separation of underivatized amino acids.

Mixed-acidic cation-exchange (MCX) columns with both strongly (SCX) and weakly (WCX) acidic functional groups were developed for the separation of standard amino acids. The resins were prepared by carboxylation of highly crosslinked monodisperse poly(styrene-divinylbenzene) copolymer particles with performic acid and subsequent sulfonation with sulfuric acid. The degree of functionalization was varied independently for each processing step and controlled by measuring pH dependent retention of the obtained resins.