Publications by authors named "Marina D Sazonova"
Article Synopsis
- * Using PCR and pyrosequencing, researchers identified various mtDNA mutations, noting four proatherogenic (harmful) and three antiatherogenic (protective) mutations.
- * The study also revealed a geographic pattern in the distribution of certain mutations, highlighting a west-east gradient in specific mtDNA mutations associated with atherosclerosis.
Background: Cells of different human organs and tissues contain different numbers of mitochondria. In these organelles, there are different copies of the mitochondrial genome, which is characteristic of a certain organ or tissue.
Objective: The aim of the investigation was to analyze the results of scientific works dedicated to the analysis of heteroplasmy levels of mitochondrial genome mutations in a number of organs and tissues.
Aim: The aim of this work was to study the effect of telomere length in the chromosomes of nuclear blood cells in individuals with coronary heart disease (CHD) on the development of cardiovascular complications (CVC).
Materials And Methods: DNA was isolated from nuclear blood cells of 498 study participants. The telomere length was determined by real-time polymerase chain reaction.
Chronic stress is a combination of nonspecific adaptive reactions of the body to the influence of various adverse stress factors which disrupt its homeostasis, and it is also a corresponding state of the organism's nervous system (or the body in general). We hypothesized that chronic stress may be one of the causes occurence of several molecular and cellular types of stress. We analyzed literary sources and considered most of these types of stress in our review article.
View Article and Find Full Text PDFBackground: The present review article considers some chronic diseases of vascular and metabolic genesis, the causes of which may be mitochondrial dysfunction. Very often, in the long course of the disease, complications may occur, leading to myocardial infarction or ischemic stroke and, as a result, death. In particular, a large percentage of human deaths nowadays belongs to cardiovascular diseases, such as coronary heart disease (CHD), arterial hypertension, cardiomyopathies, and type 2 diabetes mellitus.
View Article and Find Full Text PDFArticle Synopsis
- - The study aimed to link mitochondrial DNA (mtDNA) mutations to carotid intima-media thickness (cIMT), which helps identify individuals at high risk for cardiovascular disease, using a sample of 468 subjects from Novosibirsk.
- - Several mtDNA mutations were found to positively or negatively correlate with cIMT, highlighting their potential role as predictors for atherosclerosis development, which could improve cardiovascular risk assessments.
- - The findings suggest key predictors for cIMT variability include age, blood pressure, cholesterol levels, and specific mtDNA mutations, potentially paving the way for gene therapy targeting atherosclerosis.
In the present work, a pilot creation of four cybrid cultures with high heteroplasmy level was performed using mitochondrial genome mutations m.12315G>A and m.1555G>A.
View Article and Find Full Text PDFObjective: In this review article, we analyzed the literature on the creation of cultures containing mutations associated with cardiovascular diseases (CVD) using transfection, transduction and editing of the human genome.
Methods: We described different methods of transfection, transduction and editing of the human genome, used in the literature.
Results: We reviewed the researches in which the creation of сell cultures containing mutations was described.