Heterotrophic Microbiota from the Oligotrophic Waters of Lake Vostok, Antarctica.

Lake Vostok is the deepest lake of Antarctica but has poor accessibility for study due to a thick glacial cover, however, water samples of this lake have become available for study just recently. Previously, only the microbiome of the ice cover samples was characterized. Here we report results of bacteriological seeding with subsequent identification of the heterotrophic microorganisms (bacteria and micellar fungi) present by 16S rDNA sequencing as well as results of a direct molecular study of the water microbiome.

Discrimination of Strains from Coagulase-Negative Staphylococci and Other Pathogens by Fourier Transform Infrared Spectroscopy.

is an extremely infectious and malignant pathogen among many bacteria species. The aim of this work is to provide a robust classification model that would be able to identify independent of the culture growth stage and the variations in bacteria concentration in suspension and also one that would be able to identify the pathogen among both taxonomically close species of the same genus and taxonomically distant species of different genera, using Fourier transform infrared spectroscopy (FTIR). In total, the spectra of 141 isolates of 17 bacteria have been used.

Correction: The selective hydrosilylation of norbornadiene-2,5 by monohydrosiloxanes.

[This corrects the article DOI: 10.1039/C9RA06784A.].

The selective hydrosilylation of norbornadiene-2,5 by monohydrosiloxanes.

A simple one-step approach for the selective synthesis of -norbornenes with organosilicon substituents is suggested through the direct hydrosilylation of norbornadiene-2,5 with chlorine-free silanes. Using the example of norbornadiene-2,5 hydrosilylation with pentamethyldisiloxane and 1,1,1,3,5,5,5-heptamethyltrisiloxane, the possibility of obtaining -isomers of norbornenes with 100 -/-selectivity is shown. The investigation of Pt-, Rh-, and Pd-complexes in combination with various ligands as catalysts was performed.

Impact of recombination on polymorphism of genes encoding Kunitz-type protease inhibitors in the genus Solanum.

Background: The group of Kunitz-type protease inhibitors (KPI) from potato is encoded by a polymorphic family of multiple allelic and non-allelic genes. The previous explanations of the KPI variability were based on the hypothesis of random mutagenesis as a key factor of KPI polymorphism.

Results: KPI-A genes from the genomes of Solanum tuberosum cv.