Publications by authors named "Marilia Garcia de-Oliveira"

Studies about thymic B cells are scarce in the literature, but it was suggested that they can exert modulatory and regulatory functions on the immune system. Thymic B cells can play some role in regulating the most frequent allergic background worldwide, the atopy induced by the mite Dermatophagoides pteronyssinus (Der p). Here, we aimed to evaluate if the polyclonal IgG repertoire produced by Der p-atopic individuals can influence the homing and cytokine profile of human thymic B derived from non-atopic children aged less than seven days.

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  • COVID-19 has led to over a million deaths globally, with significant gaps in understanding its neurological effects, despite known lung disease mechanisms.
  • The study analyzed changes in brain cells (astrocytes) from Syrian hamsters infected with SARS-CoV-2, revealing disruptions in metabolic processes related to carbon metabolism and glycolysis, linked to neurological disorders.
  • Findings indicate that SARS-CoV-2 affects brain regions like the hippocampus and cortex, potentially leading to neurological symptoms such as memory loss and cognitive impairment in infected individuals.
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  • Mesenchymal stem cells (MSCs) are undifferentiated cells found in adult tissues that show promise for treating various diseases due to their ability to renew and differentiate into different cell types while modulating immune responses.
  • This study focused on human tubal mesenchymal stem cells (htMSCs) and their effects in an experimental autoimmune encephalomyelitis (EAE) model, revealing their ability to suppress the activation of dendritic cells and promote anti-inflammatory cytokine release.
  • Results indicated that htMSCs lead to milder disease symptoms, with reduced immune cell infiltration and increased levels of protective factors, suggesting their potential as a therapy for inflammatory and neurodegenerative conditions.
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Cellular therapy with mesenchymal stem cells (MSCs) is a huge challenge for scientists, as little translational relevance has been achieved. However, many studies using MSCs have proved their suppressive and regenerative capacity. Thus, there is still a need for a better understanding of MSCs biology and the establishment of newer protocols, or to test unexplored tissue sources.

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The mechanisms through which maternal immunization can modulate offspring thymic maturation of lymphocytes are not fully understood. Here, we aimed to evaluate whether maternal OVA-immunization can inhibit the maturation of Th17 cells on offspring thymus. C57BL/6 females were immunized with OVA in Alum or Alum alone and mated with normal WT males.

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Regulatory B (B10) cells can control several inflammatory diseases, including allergies; however, the origin of peripheral B10 cells is not fully understood, and the involvement of primary lymphoid organs (PLOs) as a primary site of maturation is not known. Here, using a murine model of allergy inhibition mediated by maternal immunization with ovalbumin (OVA), we aimed to evaluate whether B10 cells can mature in the thymus and whether IgG can mediate this process. Female mice were immunized with OVA, and offspring thymus, bone marrow, spleen, lung, and serum samples were evaluated at different times and after passive transfer of purified IgG or thymocytes.

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Introduction: In the last few years our group has been studying the mechanisms involved in the inhibition of allergy in offspring mediated by preconception maternal immunization, but these mechanisms are not fully understood. Such mechanisms that we have studied aimed at the passive transfer of maternal antibodies and its influence on offspring immune status.

Aim Of The Study: To evaluate whether maternal immunization could modulate intracellular Th1/Th2 profiles in offspring.

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The regulation of offspring allergy development mediated by maternal immunization was evidenced by several groups, and this mechanism seems to involve the induction of regulatory T cells (Tregs) on offspring. Here, we aimed to evaluate whether the effect of maternal immunization on offspring Tregs occurs as a result of peripheral or central modulation. Briefly, C57BL/6 female mice were immunized with OVA in Alum or Alum alone and boosted with OVA in saline or saline only after 10 and 20 days.

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Background: IL-17-producing B cells can be identified in both mice and human and were named B17 cells. The role of B17 cells still needs to be elucidated and its inflammatory or regulatory functions remain controversial.

Objective: We evaluate the effect of maternal immunization with OVA on offspring B cells that produces IL-17 and can show a regulatory potential by IL-10 production.

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The regulatory effect of allergic responses induced by IgG antibodies on human intra-thymic cells has not been reported in the literature. The aim of this study was to evaluate the possible differential effect of purified IgG from atopic and non-atopic individuals on human intra-thymic αβT cell cytokine production. Thymic tissues were obtained from 14 patients who were less than 7 d old.

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Background: The mechanisms through which allergies can be inhibited after preconception immunization with allergens are not fully understood. We aimed to evaluate whether maternal immunization can induce a regulatory B (B10) cell population in offspring in concert with allergy inhibition.

Methods: C57BL/6 females were or were not immunized with OVA and were mated with normal WT males.

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Background: Over the last decade, our group has demonstrated that murine preconception immunization with allergens has a protective effect on allergy development in offspring. The murine model used in the present study allowed us to compare allergy induction by ovalbumin (OVA) and dust mite extract from Dermatophagoides pteronyssinus (Dp).

Findings: Female mice were immunized with OVA or Dp.

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