Publications by authors named "Mariko Tsuruda"

Hematopoietic stem cells (HSCs) develop from hemogenic endothelial cells (HECs) during mouse embryogenesis. Understanding the signaling molecules required for HSC development is crucial for the in vitro derivation of HSCs. We previously induced HSCs from embryonic HECs, isolated at embryonic day 10.

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Hematopoietic stem cells (HSCs) develop from hemogenic endothelial cells (HECs) in vivo during mouse embryogenesis. When cultured in vitro, cells from the embryo phenotypically defined as pre-HSC-I and pre-HSC-II have the potential to differentiate into HSCs. However, minimal factors required for HSC induction from HECs have not yet been determined.

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Hematopoietic stem cell (HSC)-independent hematopoiesis from hemogenic endothelial cells (HECs) in the mouse embryo has been recognized as a source of tissue-resident hematopoietic cells in adult mice. Connective tissue mast cells (MCs) have been reported to originate from VE-cadherin (VE-cad)-expressing HECs in the yolk sac and embryo proper (EP) by a VE-cad-Cre-mediated lineage-tracing analysis. However, it remains unclear whether MCs are generated via a conventional HSC-dependent hematopoietic differentiation pathway, or whether through a fast-track pathway bypassing the emergence of HSCs.

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Definitive hematopoietic cells develop from fetal liver kinase 1 (Flk1) mesodermal cells during the in vitro differentiation of mouse embryonic stem cells (ESCs). VE-cadherinCD41CD45(V4145) hemogenic endothelial cells (HECs) and VE-cadherinCD41CD45 (V4145) cells mediate the definitive hematopoietic development from Flk1 cells. Bone morphogenetic protein 4 (BMP4) is known to be essential for the formation of mesoderm.

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