Publications by authors named "Marije Kuipers"
A successful mass spectrometry-based phosphoproteomics analysis relies on effective sample preparation strategies. Suspension trapping (S-Trap) is a novel, rapid, and universal method of sample preparation that is increasingly applied in bottom-up proteomics studies. However, the performance of the S-Trap protocol for phosphoproteomics studies is unclear.
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- Schistosomes can live for years in mammalian hosts by releasing products that alter the host's immune response, with these products largely being glycosylated molecules that engage specific host cell receptors called C-type lectin receptors (CLRs).
- The study focused on extracellular vesicles (EVs) from adult schistosomes, revealing that their main glycan type is LacDiNAc, while EVs from the juvenile stage (schistosomula) are highly fucosylated and primarily interact with the DC-SIGN receptor.
- The research highlights that adult worm EVs primarily bind to macrophage galactose-type lectin (MGL), indicating different glycosylation profiles and suggesting that each life stage of
Article Synopsis
- Research on extracellular vesicles (EVs) and their role in cross-species communication has gained momentum, particularly with the influence of parasitic helminths on host immune responses.
- Helminth-derived EVs are recognized as key players in these interactions, but the study of these vesicles faces unique challenges not found in mammalian models.
- To address these challenges, the authors propose best practices and a set of guidelines for the helminth research community, aiming to complement existing frameworks like MISEV and enhance understanding in the field.
Article Synopsis
- Interest in helminth-derived extracellular vesicles (EVs) has surged due to their impact on how parasites interact with their hosts, but obtaining these EVs is challenging due to limited availability and culturing options.
- The study focused on improving the purity, concentration, and yield of EVs isolated from schistosomula and adult worms using small iodixanol density gradients, which proved more effective than larger gradients.
- Results indicated that iodixanol not only allowed for faster separation of EVs from contaminants but also achieved higher yields, making it a better choice for isolating EVs from helminths and other difficult sources.
Extracellular Vesicles (EVs) are an integral component of cellular/organismal communication and have been found in the excreted/secreted (ES) products of both protozoan and metazoan parasites. Within the blood fluke schistosomes, EVs have been isolated from egg, schistosomula, and adult lifecycle stages. However, the role(s) that EVs have in shaping aspects of parasite biology and/or manipulating host interactions is poorly defined.
View Article and Find Full Text PDFPolyunsaturated fatty acids (PUFAs) and their metabolites are potent regulators of inflammation. Generally, omega ()-3 PUFAs are considered proresolving whereas -6 PUFAs are classified as proinflammatory. In this study, we characterized the inflammatory response in murine peritonitis and unexpectedly found the accumulation of adrenic acid (AdA), a poorly studied -6 PUFA.
View Article and Find Full Text PDFHelminths like release excretory/secretory (E/S) products that modulate host immunity to enable infection. Extracellular vesicles (EVs) are among these E/S products, yet molecular mechanisms and functionality of EV interaction with host immune cells is unknown. Here we demonstrate that EVs released by schistosomula are internalised by human monocyte-derived dendritic cells (moDCs).
View Article and Find Full Text PDFRecently, the interest in extracellular vesicles (EVs) released by pathogens like bacteria, fungi, and parasites has rapidly increased. Many of these pathogens actively modulate the immune responses of their host and there is accumulating evidence that pathogen-derived EV contribute to this process. The effects of pathogen-derived EV on the host immune system have been attributed to proteins, lipids, nucleic acids, and glycans contained in, or present on these EV.
View Article and Find Full Text PDFphototransduction is mediated by phospholipase C, leading to activation of transient receptor potential (TRP) and TRP-like (TRPL) channels by mechanisms that are unresolved. A role for InsP receptors (IPRs) had been excluded because IPR mutants () appeared to have normal light responses; however, this was recently challenged by Kohn et al. ("Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of photoreceptors in vivo," 35:2530), who reported defects in phototransduction after IPR-RNAi knockdown.
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