CNS tumors with PLAGL1-fusion: beyond ZFTA and YAP1 in the genetic spectrum of supratentorial ependymomas.

A novel methylation class, "neuroepithelial tumor, with PLAGL1 fusion" (NET-PLAGL1), has recently been described, based on epigenetic features, as a supratentorial pediatric brain tumor with recurrent histopathological features suggesting an ependymal differentiation. Because of the recent identification of this neoplastic entity, few histopathological, radiological and clinical data are available. Herein, we present a detailed series of nine cases of PLAGL1-fused supratentorial tumors, reclassified from a series of supratentorial ependymomas, non-ZFTA/non-YAP1 fusion-positive and subependymomas of the young.

CNS erythroblastic sarcoma: a potential emerging pediatric tumor type characterized by NFIA::RUNX1T1/3 fusions.

Erythroblastic sarcoma (ES) (previously called chloroma or granulocytic sarcoma) are rare hematological neoplams characterized by the proliferation of myeloid blasts at extramedullary sites, and primarily involve the skin and soft tissue of middle-aged adults. ES may be concomitant with or secondary to myeloid neoplasms (mostly acute myeloid leukemia (AML)) or in isolated cases (de novo) without infiltration of the bone marrow by blasts. ES share cytogenetic and molecular abnormalities with AML, including RUNX1T1 fusions.

The pontine diffuse midline glioma, EGFR-subtype with ependymal features: Yet another face of diffuse midline glioma, H3K27-altered.

Diffuse midline glioma with two components: classical glial and ependymal.

CNS tumor with EP300::BCOR fusion: discussing its prevalence in adult population.

The Central Nervous System (CNS) tumor with BCOR internal tandem duplication (ITD) has recently been added as a novel embryonal histomolecular tumor type to the 2021 World Health Organization (WHO) Classification of CNS Tumors. In addition, other CNS tumors harboring a BCOR/BCORL1 fusion, which are defined by a distinct DNA-methylation profile, have been recently identified in the literature but clinical, radiological and histopathological data remain scarce. Herein, we present two adult cases of CNS tumors with EP300::BCOR fusion.

Posterior fossa ependymoma H3 K27-mutant: an integrated radiological and histomolecular tumor analysis.

Posterior fossa group A ependymomas (EPN_PFA) are characterized by a loss of H3 K27 trimethylation due to either EZHIP overexpression or H3 p.K27M mutation, similar to H3 K27-altered diffuse midline gliomas (DMG), but in reverse proportions. Very little data is available in the literature concerning H3 K27M-mutant EPN_PFA.

Separation and preconcentration of actinides from concentrated nitric acid by extraction chromatography in microsystems.

An original method of monolith impregnation in microsystem for the analysis of radionuclides in nitric acid is reported. Three microcolumns made of monolith poly(AMA-co-EDMA) were impregnated in COC microsystems. The robustness of the microsystems in nitric acid media until 8 M was demonstrated.

Two approaches for estimating the lower limit of quantitation (LLOQ) of microRNA levels assayed as exploratory biomarkers by RT-qPCR.

Background: Circulating microRNAs are undergoing exploratory use as safety biomarkers in drug development. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is one common approach used to quantitate levels of microRNAs in samples that includes the use of a standard curve of calibrators fit to a regression model. Guidelines are needed for setting assay quantitation thresholds that are appropriate for this method and to biomarker pre-validation.

Absolute Measurement of Cardiac Injury-Induced microRNAs in Biofluids across Multiple Test Sites.

Extracellular microRNAs (miRNAs) represent a promising new source of toxicity biomarkers that are sensitive indicators of site of tissue injury. In order to establish reliable approaches for use in biomarker validation studies, the HESI technical committee on genomics initiated a multi-site study to assess sources of variance associated with quantitating levels of cardiac injury induced miRNAs in biofluids using RT-qPCR. Samples were generated at a central site using a model of acute cardiac injury induced in male Wistar rats by 0.

Potential of ion imprinted polymers synthesized by trapping approach for selective solid phase extraction of lanthanides.

Ion imprinted polymers (IIPs) specific to lanthanides were synthesized using neodymium ions (Nd) as template ions. Nd ions form binary complex ions with 5,7-dichloroquinoline-8-ol (DCQ) or vinylpyridine (VP), or ternary complex ions with both DCQ and VP in 2-methoxyethanol, before copolymerization in the presence of styrene and divinylbenzene as monomer and cross-linker, respectively. DCQ was expected to be trapped in the synthesized polymers pores.

Correction: Identification of microRNAs in Macaca fascicularis (Cynomolgus Monkey) by Homology Search and Experimental Validation by Small RNA-Seq and RT-qPCR Using Kidney Cortex Tissues.

[This corrects the article DOI: 10.1371/journal.pone.

Photochemical Synthesis and Versatile Functionalization Method of a Robust Porous Poly(ethylene glycol methacrylate-co-allyl methacrylate) Monolith Dedicated to Radiochemical Separation in a Centrifugal Microfluidic Platform.

The use of a centrifugal microfluidic platform is an alternative to classical chromatographic procedures for radiochemistry. An ion-exchange support with respect to the light-addressable process of elaboration is specifically designed to be incorporated as a radiochemical sample preparation module in centrifugal microsystem devices. This paper presents a systematic study of the synthesis of the polymeric porous monolith poly(ethylene glycol methacrylate-co-allyl methacrylate) used as a solid-phase support and the versatile and robust photografting process of the monolith based on thiol-ene click chemistry.

Identification of microRNAs in Macaca fascicularis (Cynomolgus Monkey) by Homology Search and Experimental Validation by Small RNA-Seq and RT-qPCR Using Kidney Cortex Tissues.

MicroRNAs (miRNAs) present in tissues and biofluids are emerging as sensitive and specific safety biomarkers. MiRNAs have not been thoroughly described in M. fascicularis, an animal model used in pharmaceutical industry especially in drug safety evaluation.

Liquid-liquid extraction of uranium(VI) with Aliquat® 336 from HCl media in microfluidic devices: Combination of micro-unit operations and online ICP-MS determination.

The analysis of radionuclides is carried out according to operating protocols which include a series of chemical operations such as separation/purification steps and therefore is usually time-consuming, complex and difficult to put in place in a hostile environment (gloves box). In the present work, the liquid-liquid extraction of uranium in HCl media by Aliquat® 336 was performed in microsystem to demonstrate the potential of miniaturization in radiochemistry compared to classical protocols (decrease in volumes, analysis time, radiations received and feasibility of automation). Through the investigation of the influence of different parameters (flow rates, length of the microchannel, specific interfacial area), it was shown that the same performances of liquid-liquid extraction can be obtained in microsystem and in batch.

Centrifugal microfluidic platform for radiochemistry: potentialities for the chemical analysis of nuclear spent fuels.

The use of a centrifugal microfluidic platform is for the first time reported as an alternative to classical chromatographic procedures for radiochemistry. The original design of the microfluidic platform has been thought to fasten and simplify the prototyping process with the use of a circular platform integrating four rectangular microchips made of thermoplastic. The microchips, dedicated to anion-exchange chromatographic separations, integrate a localized monolithic stationary phase as well as injection and collection reservoirs.

Analysis of twenty five impurities in uranium matrix by ICP-MS with iron measurement optimized by using reaction collision cell, cold plasma or medium resolution.

An analytical procedure was developed to determine the concentration of 25 impurities (Li, Be, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Zr, Mo, Ag, Cd, In, Sm, Eu, Gd, Dy, W, Pb, Bi and Th) in a uranium matrix using the quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS). The dissolution of U(3)O(8) powder was made with a mixture of hydrochloric acid and nitric acid. Then, a selective separation of uranium using the UTEVA column was used before measurement by Q-ICP-MS.

Synthesis and characterization of ammonium functionalized porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths for microscale analysis and its application to DNA purification.

A systematic study of parameters affecting the nucleophilic addition of secondary and tertiary amines on reactive epoxy groups was conducted on porous polymer monoliths. Reaction of small amines like diethylamine (DEA) or triethylamine (TEA) on poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths (poly(GMA-co-EDMA)) allows to prepare anion exchange media. This study aimed to determine optimal and suitable conditions to prepare anion-exchange porous monolith inside 100 microm internal diameter capillary.

Improved chromatographic performances of glycidyl methacrylate anion-exchange monolith for fast nano-ion exchange chromatography.

An efficient and reproducible photopolymerized poly(glycidyl methacrylate-co-ethylene dimethacrylate) was synthesized in Teflon-coated fused-silica capillaries (100 μm id) and functionalized by reaction of triethylamine with reactive epoxy groups. We report here the successful transfer of a standard polymerization mixture optimized for the thermally initiated synthesis of glycidyl-based monolith to photo-induced polymerization. The monolith obtained after optimization of the photo-initiation conditions was characterized in reverse-phase chromatography evaluating its suitability in terms of efficiency, retention and hydrodynamic permeability.

Heavy metal bioaccumulation by the bryophyte Scleropodium purum at three French sites under various influences: rural conditions, traffic, and industry.

An active biomonitoring of the heavy metals pollution experiment was undertaken by means of the bryophyte species Scleropodium purum transplanted at three different sites exposed to rural, traffic, or industrial influences. Concentrations of about 40 elements in S. purum were determined by instrumental neutron activation analysis and inductively coupled plasma mass spectrometry.

Evidence of double-walled Al-Ge imogolite-like nanotubes. a cryo-TEM and SAXS investigation.

It has been recently discovered that the synthesis of Al-Ge imogolite-like nanotubes is possible at high concentration. Despite this initial success, the structure of these Al-Ge imogolite-like nanotubes remains not completely understood. Using high resolution cryo-TEM and Small Angle X-ray Scattering, we unravel their mesoscale structure in two contrasted situations.

Ripening of catanionic aggregates upon dialysis.

We have studied the dialysis of surfactant mixtures of two oppositely charged surfactants (catanionic mixture) by combining HPLC, neutron activation, confocal microscopy, and NMR. In mixtures of n-alkyl trimethylammonium halides and n-fatty acids, we have demonstrated the existence of a specific ratio between both surfactant contents (anionic/cationic almost equal to 2:1) that determines the morphology, the elimination of ions, and the elimination of the soluble cationic surfactant upon dialysis. In mixtures prepared with lower anionic surfactant contents, ill-defined aggregates are formed, and dialysis quickly eliminates the ion pairs (H+X-) formed upon surfactant association and also the cationic surfactant until a limiting 2:1 ratio is reached.

Relevance of NH4F in acid digestion before ICP-MS analysis.

In order to implement a simpler, less expensive and more safe sample dissolution procedure, we have substituted the HF-HClO(4) mixture by NH(4)F. By testing three certified reference materials, lichen 336, basalt BE-N, soil 7, it was found that the three-reagents digestion without HF and HClO(4) (HNO(3)+H(2)O(2)+NH(4)F was used) was very effective for the pretreatment of ICP-MS measurement. The comparison was based on the measurement results and their uncertainties.

A PIXE and ICP-MS analysis of metallic atmospheric contaminants in tree bark tissues, a basis for biomonitoring uses.

The qualitative and quantitative metallic content of tree barks of Argania spinosa (L.) Skeels were studied. Argania spinosa is an endemic species in Morocco.