Publications by authors named "Mariem Saidani"
ESBL- and mcr-1-producing Escherichia coli in veal calves in Tunisia.
J Glob Antimicrob Resist
December 2019
Article Synopsis
- Enterobacteriaceae resistant to extended-spectrum cephalosporins (ESC-R) are a significant health concern, especially related to potential transmission to humans through the food chain, with the chicken industry being a major focus.
- A study in Tunisia collected 258 cloacal swabs from five different chicken farms, all of which tested positive for ESC-R, showing prevalence rates between 4% and 67.3%.
- The research identified a dominant ESC-R gene/plasmid combination (IncI1/ST3) in chickens, alongside various other resistance genes, suggesting a high diversity of resistant bacteria that could spread through the food supply.
Objectives: Resistance to extended-spectrum cephalosporins, fluoroquinolones and colistin is under constant scrutiny in food-producing animals worldwide. However, little is known about camels, which provide milk and meat for human consumption, and are attractions for tourists to ride in arid regions. This study assessed the role of camels as potential reservoirs of these resistance determinants.
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- Bovine mastitis is a significant issue in dairy farming, leading to substantial economic losses, with major pathogens including Staphylococci, Streptococci, and coliforms.
- This study highlights the prevalence of Escherichia coli (31.7%) and Klebsiella pneumoniae (20.7%) as key contributors to mastitis in African dairy cattle, alongside important resistance findings to antibiotics such as tetracycline and sulfonamides.
- It emphasizes the urgent need for improved farm management practices in Tunisia to manage coliform-related mastitis and curb the spread of antibiotic-resistant strains that pose risks to public health.
In cattle, anaplasmosis is a tick-borne rickettsial disease caused by Anaplasma marginale, A. centrale, A. phagocytophilum, and A.
View Article and Find Full Text PDFTo date, there have been no reports on seasonal variations of Anaplasma spp. in South Mediterranean small ruminants. In this longitudinal field study, single and mixed Anaplasma spp.
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- Molecular diagnosis of Anaplasma platys in carnivores and ruminants is complicated by co-infections, necessitating a new method combining a RFLP assay with hemi-nested PCR of the groEL gene.
- A study on 963 domesticated ruminants in North Tunisia found varying infection rates of A. platys-like, with significant prevalence in goats (22.8%) and lower rates in sheep (11%) and cattle (3.5%).
- The developed RFLP assay provides a faster, more reliable detection tool for A. platys and related strains, facilitating epidemiological studies and improving the specificity of identifying different Anaplasma species in hosts.
Accurate diagnosis of animal and zoonotic diseases, such as granulocytic anaplasmosis, is crucial to estimate risk during control programs. In this study, 16S rRNA nested PCR and RFLP assay were combined to investigate the presence of Anaplasma phagocytophilum and genetically related strains (namely A. phagocytophilum-like 1 and 2) in 936 Tunisian ruminants.
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