Publications by authors named "Marielton P Cunha"
Article Synopsis
- The study explores the resurgence of Oropouche fever in Brazil from 2023 to 2024, focusing on virological factors influencing the outbreak after decades of sporadic cases.
- Researchers gathered data from various sources, analyzed serum samples from patients and previously infected individuals, and performed molecular tests to understand the virus's characteristics and behavior.
- The findings showed an extraordinary spike in cases in 2024, with over 8,600 confirmed incidents, highlighting the virus's widespread distribution across all regions of Brazil.
Article Synopsis
- * The study included data from 2015 to 2024, revealing a dramatic rise in infections—83.2% occurring in the North—and identified a novel OROV strain that is much more virulent than the original prototype.
- * The new OROV strain was shown to replicate significantly faster and more effectively in mammalian cells, with a notable decrease in neutralizing antibodies from individuals previously infected, indicating a potential threat to public health.
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). "Extraction-less" or "direct" real time-reverse transcription polymerase chain reaction (RT-PCR) is a transparent and accessible qualitative method for SARS-CoV-2 detection from nasopharyngeal or oral pharyngeal samples with the potential to generate actionable data more quickly, at a lower cost, and with fewer experimental resources than full RT-qPCR. This study engaged 10 global testing sites, including laboratories currently experiencing testing limitations due to reagent or equipment shortages, in an international interlaboratory ring trial.
View Article and Find Full Text PDFReverse transcription-quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). "Extraction-less" or "direct" real time-reverse transcription polymerase chain reaction (RT-PCR) is an open-access qualitative method for SARS-CoV-2 detection from nasopharyngeal or oral pharyngeal samples with the potential to generate actionable data more quickly, at a lower cost, and with fewer experimental resources than full RT-qPCR. This study engaged 10 global testing sites, including laboratories currently experiencing testing limitations due to reagent or equipment shortages, in an international interlaboratory ring trial.
View Article and Find Full Text PDFArticle Synopsis
- The study monitored Zika virus (ZIKV) in four healthy adults for up to 298 days post-symptoms, focusing on virus presence in different body fluids and immune response.
- The two male patients shed ZIKV for up to 158 days, with virus detected in sperm cells, indicating potential sexual transmission, yet one was able to conceive afterward.
- Genetic analysis showed changes in the virus over time and suggested separate strains in the reproductive and urinary systems, highlighting risks of long-term virus presence in the male reproductive system.
Yellow fever virus RNA is usually detected in blood of infected humans. We detected virus RNA in urine and semen samples from a convalescent patient. A complete virus genome was sequenced for an isolate from a urine sample.
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