Objective measurement of postocclusion surge during phacoemulsification in human eye-bank eyes.

Purpose: To objectively compare the postocclusion vacuum surge among different phacoemulsification machines and devices.

Design: Experimental study.

Methods: Infiniti, Legacy, Millennium, and Sovereign were tested in an eye-bank eye.

Survey of intensive care physicians on the recognition and management of intra-abdominal hypertension and abdominal compartment syndrome.

Objective: To assess current understanding and clinical management of intra-abdominal hypertension and abdominal compartment syndrome among critical care physicians.

Design: A ten-question, written survey.

Setting: University health sciences center.

Phacoemulsification tip vacuum pressure: Comparison of 4 devices.

Purpose: To determine the vacuum pressure generated by 4 phacoemulsification devices measured at the phacoemulsification tip.

Setting: University ophthalmology department.

Methods: The effective vacuum pressures generated by the Sovereign (AMO), Millennium (Bausch & Lomb), Legacy AdvanTec (Alcon Laboratories), and Infiniti (Alcon Laboratories) phacoemulsification machines were measured with a device that isolated the phacoemulsification tip in a chamber connected to a pressure gauge.

Thermal inertia associated with ultrapulse technology in phacoemulsification.

Purpose: To determine whether very short pulses of ultrasound (5 to 6 milliseconds) have less heat propagation in biological tissue (thermal inertia) than traditional pulses (50 milliseconds).

Setting: Department of Ophthalmology and Visual Sciences, University of Utah Health Sciences Center, Salt Lake City, Utah.

Methods: Thermal testing was done in balanced salt solution (BSS) and in eye-bank eyes.

Dominant optic atrophy, sensorineural hearing loss, ptosis, and ophthalmoplegia: a syndrome caused by a missense mutation in OPA1.

Purpose: To describe the clinical features of and identify the disease-causing mutation in a large Utah family segregating a dominantly inherited syndrome of optic atrophy, sensorineural hearing loss, ptosis, and ophthalmoplegia.

Design: Observational case series.

Methods: Thirty individuals at risk for a syndrome of optic atrophy, sensorineural hearing loss, ptosis, and ophthalmoplegia in a single family underwent clinical examinations and venipuncture.

Ubiquitin interactions of NZF zinc fingers.

Ubiquitin (Ub) functions in many different biological pathways, where it typically interacts with proteins that contain modular Ub recognition domains. One such recognition domain is the Npl4 zinc finger (NZF), a compact zinc-binding module found in many proteins that function in Ub-dependent processes. We now report the solution structure of the NZF domain from Npl4 in complex with Ub.

HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein.

The HIV-1 Gag protein recruits the cellular factor Tsg101 to facilitate the final stages of virus budding. A conserved P(S/T)AP tetrapeptide motif within Gag (the "late domain") binds directly to the NH2-terminal ubiquitin E2 variant (UEV) domain of Tsg101. In the cell, Tsg101 is required for biogenesis of vesicles that bud into the lumen of late endosomal compartments called multivesicular bodies (MVBs).

Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4.

Ubiquitylated proteins are directed into a large number of different cellular pathways through interactions with effector proteins that contain conserved ubiquitin binding motifs. Here, we report the solution structure and ubiquitin binding properties of one such motif, the Npl4 zinc finger or RanBP2/Nup358 zinc finger (NZF) domain. Npl4 NZF forms a compact module composed of four antiparallel beta-strands linked by three ordered loops.