Publications by authors named "Marielle Bedotto-Buffet"

Article Synopsis
  • Acute respiratory infections are a major global health issue, and more research is needed on the microorganisms causing these infections in various regions, particularly in Senegal.
  • In a study of 500 febrile patients, researchers found that 73.2% had at least one detectable microorganism, with viruses and bacteria commonly identified, including influenza and RSV.
  • The presence of certain microorganisms was linked to respiratory symptoms, and co-infections were also associated with more severe symptoms, highlighting the need for better diagnostic tools to adapt treatment strategies.
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Since the onset of the COVID-19 pandemic, the SARS-CoV-2 viral dynamics in Africa have been less documented than on other continents. In Gabon, a Central African country, a total number of 37,511 cases of COVID-19 and 281 deaths have been reported as of December 8, 2021. After the first COVID-19 case was reported on March 12, 2020, in the capital Libreville, the country experienced two successive waves.

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Background: The SARS-CoV-2 pandemic has impacted tissue transplantation procedures since conjunctivas were found to be associated with coronavirus infection. Here, we investigated infection of a cornea graft from a COVID-19-positive donor.

Methods: In order to evaluate the presence of SARS-CoV-2 in the cornea graft we first carried out a qRT-PCR and then we investigated the presence of SARS-CoV-2 by fluorescence and electron microscopy.

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Background: The PCR-based detection of archaea DNA in human specimens relies on efficient DNA extraction. We previously designed one such protocol involving only manual steps. In an effort to reduce the workload involved, we compared this manual protocol to semi-automated and automated protocols for archaea DNA extraction from human specimens.

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