The digestive tract sections of the sea cucumber Isostichopus badionotus reveal differences in composition, diversity, and functionality of the gut microbiota.

For the first time, this study analyses the composition and diversity of the gut microbiota of Isostichopus badionotus in captivity, using high-throughput 16S rRNA sequencing, and predicts the metagenomic functions of the microbiota. The results revealed a different composition of the gut microbiota for the foregut (FG) and midgut (MG) compared to the hindgut (HG), with a predominance of Proteobacteria, followed by Actinobacteria, Bacteroidetes, and Firmicutes. The FG and MG demonstrated a greater bacterial diversity compared to the HG.

Effect of the use of Bacillus spp. on the characteristics of dissolved fluorescent organic matter and the phytochemical quality of Stevia rebaudiana grown in a recirculating aquaponic system.

The effect of the incorporation of mineralizing Bacillus spp. on the characteristics of fluorescent organic matter (FDOM) in a recirculating aquaculture system (Nile tilapia-Stevia rebaudiana) was evaluated. EEM-PARAFAC analysis was used to determine the composition of the dissolved organic matter and to study its relationship with nitrogen transformation.

Evaluation of two independent protocols for the extraction of DNA and RNA from different tissues of sea cucumber .

is a sea cucumber species of great ecological and economic relevance for Mexico and Central American and Caribbean countries; however, the protocols for the extraction of the nucleic acids have not yet been published. In this study, we describe the first protocols to obtain DNA and RNA from different tissues of which include the respiratory tree, gonad, longitudinal muscle bands, anterior intestine and cloaca. The extraction of high-quality DNA was performed using the DNeasy Blood & Tissue kit (Qiagen, Valencia, CA, USA) with minor modifications in different points of the protocol.

Molecular Characterization of Histamine-Producing Psychrotrophic Bacteria Isolated from Red Octopus () in Refrigerated Storage.

The present study aimed at determining the histamine production capacity of Gram (+) and Gram (-) bacteria isolated from , along with identifying the presence of amino acid decarboxylase genes. Of the total 80 psychrotrophic microorganisms, 32 strains were identified as histamine-forming bacteria. The recombinant DNA technique was used for genotypic identification of histidine (), ornithine (), and lysine decarboxylases () genes.

Integrated effect of nutrients from a recirculation aquaponic system and foliar nutrition on the yield of tomatoes Solanum lycopersicum L. and Solanum pimpinellifolium.

The objective of this study was to evaluate the potential of tomato plants to efficiently use the nitrogen (N) of a recirculation aquaponic system (RAS) and to evaluate the effects of foliar fertilization as a complement to the water nutrition on the growth of the two tomato cultivars. The significant effect of six macro- and seven micronutrients was evaluated on the plant growth and on the fruit yield. Two experiments were performed in a nutrient film aquaponic unit.

Growth kinetics of Escherichia coli O157:H7 on the epicarp of fresh vegetables and fruits.

Despite the increasing reports on the incidence of fresh vegetables and fruits as a possible vehicle for human pathogens, there is currently limited knowledge on the growth potential of Escherichia coli O157:H7 on different plant substrates. This study analyzed the selective adhesion and growth of E. coli O157:H7 on chili habanero (Capsicum chinense L.

Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds from Brosimum alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity.

In order to maximize the yield of the total phenolic content (TPC) and total monomeric anthocyanin (TMA) from leaf and to study the radical-scavenging activity, a three-level three-factor Box-Behnken design (BBD) was used to determine the optimal points for ultrasound-assisted extraction (UAE). In this study, we analyzed the extraction time (10, 20, and 30 min), temperature (28, 30, and 32 °C), and probe sonication power (40%, 28 W/cm²; 60%, 51 W/cm²; and 80%, 74 W/cm²). Analysis of variance (ANOVA) indicated that the sonication power plays a significant role in the extraction of phenolic compounds.

Effect of pH on temperature controlled degradation of reactive oxygen species, heat shock protein expression, and mucosal immunity in the sea cucumber Isostichopus badionotus.

This study evaluated the effect of pH on the activity of antioxidant and immune enzymes in the sea cucumber Isostichopus badionotus exposed to different temperatures. The organisms (530 ±110 g) were exposed to 16, 20, 24, 28, 30, 34 and 36°C for 6 h to evaluate thermal limits at two water pH values (treatment = 7.70; control = 8.

Physiological changes in the red drum after long-term freshwater acclimation.

The effect of long-term freshwater acclimation on the blood and plasma ion composition of Red Drum Sciaenops ocellatus was investigated with the goal of elucidating the necessity of ion remediation. Four replicates (n = 50) of freshwater-acclimated (FW) fish (1.6 ± 0.