Background: Fructosamine 3 kinase (FN3K) is a deglycating enzyme, which may play a key role in reducing diabetes-induced organ damage by removing bound glucose from glycated proteins. We wanted to develop a simple colorimetric method for assaying FN3K activity in human body fluids.
Methods: Glycated bovine serum albumin (BSA) was obtained by glycation with a 10% glucose solution at 37 °C.