Pyridoxamine reduces postinjury fibrosis and improves functional recovery after acute kidney injury.

Acute kidney injury (AKI) is a common and independent risk factor for death and chronic kidney disease (CKD). Despite promising preclinical data, there is no evidence that antioxidants reduce the severity of injury, increase recovery, or prevent CKD in patients with AKI. Pyridoxamine (PM) is a structural analog of vitamin B6 that interferes with oxidative macromolecular damage via a number of different mechanisms and is in a phase 3 clinical efficacy trial to delay CKD progression in patients with diabetic kidney disease.

Comparison of blood microsampling with DBS and conventional blood collection techniques used in a midazolam biostudy.

Background: Quantitative DBS LC-MS/MS assay for midazolam was used to compare two sample collection techniques (venipuncture and finger prick) and the midazolam concentrations measured in plasma samples, DBS and dried plasma spots.

Methodology: Midazolam was extracted from DBS cards and compared with whole blood collected from usual venipuncture. Dried plasma spots were also compared with plasma.

The effect of different temperatures on the fatty acid composition of Rhizobium leguminosarum bv. viciae in the faba bean symbiosis.

Fatty acid composition was determined in cells of strains CBhS and CBp7 of Rhizobium leguminosarum bv. viciae grown at four temperatures (10, 15, 22 and 30°C), and in bacteroids and nodules formed with faba bean (Vicia faba L.) grown at two day/night temperature regimes (22/15 and 15/10°C).