Publications by authors named "Marie-Adelaide Bout"
Article Synopsis
- The study investigates the effectiveness of a rapid immunoassay technique (Snibe) versus mass spectrometry (LC-MS/MS) in measuring plasma homocysteine levels, particularly in patients with nitrous oxide abuse.
- Significant elevations in plasma homocysteine were found in nitrous oxide abusers, with the immunoassay delivering faster results, although it generally underestimated high levels compared to LC-MS/MS.
- The findings highlight the need for the immunoassay as a quick initial screening tool, while still recommending confirmation with LC-MS/MS for accurate diagnosis of conditions associated with oxidative stress.
Tacrolimus (FK506) is an immunosuppressant that is experiencing a continuous rise in usage worldwide. The related side effects are known to be globally dose-dependent. Despite numerous studies on FK506, the mechanisms underlying FK506 toxicity are still not well understood.
View Article and Find Full Text PDFContext: Plasma branched chain amino acid (BCAA) concentrations correlate positively with body mass index (BMI), measures of insulin resistance (IR), and severity of nonalcoholic fatty liver disease (NAFLD). Moreover, plasma BCAA concentrations also differ between the sexes, which display different susceptibilities to cardio-metabolic diseases.
Objective: Assess whether plasma BCAA concentrations associate with NAFLD severity independently of BMI, IR, and sex.
Background: Blood polyunsaturated fatty acid (PUFA) levels are determined by diet and by endogenous synthesis via Δ5- and Δ6-desaturases (encoded by the FADS1 and FADS2 genes, respectively). Genome-wide association studies have reported associations between FADS1-FADS2 polymorphisms and the plasma concentrations of PUFAs, HDL- and LDL-cholesterol, and triglycerides. However, much remains unknown regarding the molecular mechanisms explaining how variants affect the function of FADS1-FADS2 genes.
View Article and Find Full Text PDFCreatine transporter is currently the focus of renewed interest with emerging roles in brain neurotransmission and physiology, and the bioenergetics of cancer metastases. We here report on amendments of a standard creatine uptake assay which might help clinical chemistry laboratories to extend their current range of measurements of creatine and metabolites in body fluids to functional enzyme explorations. In this respect, short incubation times and the use of a stable-isotope-labeled substrate (D-creatine) preceded by a creatine wash-out step from cultured fibroblast cells by removal of fetal bovine serum (rich in creatine) from the incubation medium are recommended.
View Article and Find Full Text PDFBackground: The repeated blackening of in-line filters has been observed during the infusion of parenteral nutrition 2-in-1 mixtures (binary parenteral nutrition [BPN]) delivered in a neonatal intensive care unit. This study aimed to examine the elemental content of precipitates isolated from infused BPN bags and determine the main physicochemical interactions occurring in these bags.
Materials And Methods: The infusion of BPN mixtures was simulated in vitro following hospital practices.
Background: Guanidinoacetate methyltransferase (GAMT) deficiency is a recently described disorder and few cases have been reported to date. As it is a treatable pathology, we seek to contribute to its better understanding, particularly to further elucidate its biochemical diagnosis for early treatment.
Methods: The patients, two brothers aged 13 years (P1) and 11 years (P2), have been explored for signs and symptoms suggestive of inborn errors of metabolism.