Issue when expressing a recombinant protein under the control of p in BY-2 cells.

Several recombinant proteins have been successfully produced in plants. This usually requires -mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery via , especially when the protein of interest is toxic to the bacteria.

In vivo deglycosylation of recombinant glycoproteins in tobacco BY-2 cells.

Production of recombinant pharmaceutical glycoproteins has been carried out in multiple expression systems. However, N-glycosylation, which increases heterogeneity and raises safety concerns due to the presence of non-human residues, is usually not controlled. The presence and composition of N-glycans are also susceptible to affect protein stability, function and immunogenicity.

Sustainable health-based soil standards for arsenic using epidemiological data and toxicokinetic/probabilistic modelling.

This study presents an alternative method to the traditional reference dose approach for the determination of health risk-based soil standards for arsenic. The model combines multimedia equations for air, soil and dietary exposure, a toxicokinetic component, a probabilistic output, a reference distribution for urine arsenic in the general population and exposure parameters values traceable in US-EPA or EFSA reference studies. The model calculates a mean inorganic As urine concentration AsU (sum of inorganic arsenic Asi and its metabolites) from environmental data and exposure parameters, which is attributed to the central value of a lognormal distribution.

Masked hypertension in obstructive sleep apnea syndrome.

Background: Ambulatory blood pressure (BP) monitoring (ABPM) detects subjects with normal clinic but high ambulatory 24-h BP, that is, masked hypertension.

Methods: One hundred and thirty newly diagnosed obstructive sleep apnea syndrome (OSAS) patients, free of recognized cardiovascular disease were included (111 men, age = 48 +/- 1 years, BMI = 27.6 +/- 0.