Advancing quantification methods for polyphenols in brown seaweeds-applying a selective qNMR method compared with the TPC assay.

Introduction: Brown seaweeds are a sustainable biomass with a potential for various industrial applications. Polyphenols are an important contributor to this potential.

Objective: The aim was total quantification of polyphenols in brown seaweeds from different tidal zones, using a selective H quantitative NMR (qNMR) method, comparing the results with the colorimetric Folin-Ciocalteu total phenolic content (TPC) assay.

Quantification of Polyphenols in Seaweeds: A Case Study of .

In this case study, we explored quantitative H NMR (qNMR), HPLC-DAD, and the Folin-Ciocalteu assay (TPC) as methods of quantifying the total phenolic content of a green macroalga, , after optimized accelerated solvent extraction. Tentative qualitative data was also acquired after multiple steps of purification. The observed polyphenolic profile was complex with low individual concentrations.