Antagonistic Effects of 090104 on Respiratory Pathogens.

In previous studies, it was demonstrated that 090104, isolated from the human nasopharynx, modulates respiratory immunity, improving protection against infections. Here, the antagonistic effect of the 090104 strain on respiratory pathogens, including , , , , and , was explored. In a series of in vitro studies, the capacity of 090104, its bacterium-like particles, and its culture supernatants to coaggregate, inhibit the growth, and change the virulent phenotype of pathogenic bacteria was evaluated.

The Mucus-Binding Factor Mediates CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract.

CRL1505 possesses immunomodulatory activities in the gastrointestinal and respiratory tracts when administered orally. Its adhesion to the intestinal mucosa does not condition its beneficial effects. The intranasal administration of CRL1505 is more effective than the oral route at modulating immunity in the respiratory tract.

Bacterium-like Particles from as Mucosal Adjuvant for the Development of Pneumococcal Vaccines.

Previously, it was shown that intranasally (i.n.) administered 090104 (Cp) or CP-derived bacterium-like particles (BLPs) improve the immunogenicity of the pneumococcal conjugate vaccine (PCV).

Immunobiotic FFIG58 Confers Long-Term Protection against .

Previously, we isolated potentially probiotic strains from the intestines of wakame-fed pigs. The strains were characterized based on their ability to modulate the innate immune responses triggered by the activation of Toll-like receptor (TLR)-3 or TLR4 signaling pathways in intestinal mucosa. In this work, we aimed to evaluate whether nasally administered strains are capable of modulating the innate immune response in the respiratory tract and conferring long-term protection against the respiratory pathogen .

In Silico Comparative Genomic Analysis Revealed a Highly Conserved Proteolytic System in .

, the type species of the genus , is widely recognized as the primary starter culture in the dairy industry due to its proteolytic activity, which enables it to growth in milk. In this study, a comprehensive genomic analysis of the proteolytic system was conducted on strains. The analysis included 27 genomes of , with a specific focus on the key enzyme involved in this system, the cell envelope-associated proteinase (CEP).

Oral Administration of CRL1505 Modulates Lung Innate Immune Response against ST25.

Orally administered CRL1505 enhances respiratory immunity, providing protection against respiratory viruses and . However, the capacity of the CRL1505 strain to improve respiratory immunity against Gram-negative bacterial infections has not been evaluated before. The aim of this work was to evaluate whether the CRL1505 was able to beneficially regulate the respiratory innate immune response and enhance the resistance to hypermucoviscous KPC-2-producing of the sequence type 25 (ST25).

Hypermucoviscous Carbapenem-Resistant ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation.

is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant with sequence type 25 (ST25) in hospitals in Norwest Argentina was observed. This work aimed to study the virulence and inflammatory potential of two ST25 strains (LABACER01 and LABACER27) in the intestinal mucosa.

The Respiratory Commensal Bacterium as a Mucosal Adjuvant for Nasal Vaccines.

Previously, we demonstrated that nasally administered 090104 (Cp) or its bacterium-like particles (BLPs) increase the resistance of mice against bacterial and viral respiratory pathogens by modulating the innate immunity. In this work, we evaluated the ability of Cp and BLPs to stimulate alveolar macrophages, and to enhance the humoral immune response induced by a commercial vaccine against . In the first set of experiments, Cp or the BLPs were incubated with primary cultures of murine alveolar macrophages and the phagocytic activity, and the production of cytokines was evaluated.

Strains Modulate Intestinal Innate Immune Response and Increase Resistance to Enterotoxigenic Infection.

Currently, probiotic bacteria with not transferable antibiotic resistance represent a sustainable strategy for the treatment and prevention of enterotoxigenic (ETEC) in farm animals. is among the most versatile species used in the food industry, either as starter cultures or probiotics. In the present work, the immunobiotic potential of CRL681 and CRL1506 was studied to evaluate their capability to improve the resistance to ETEC infection.

The Mucus Binding Factor Is Not Necessary for CRL1505 to Exert Its Immunomodulatory Activities in Local and Distal Mucosal Sites.

Both viable and non-viable orally administered CRL1505 modulate immunity in local (intestine) and distal (respiratory) mucosal sites. So, intestinal adhesion and colonization are not necessary for this probiotic strain to exert its immunomodulatory effects. In this work, a mucus-binding factor knockout CRL1505 strain (ΔCRL1505) was obtained and the lack of binding ability to both intestinal epithelial cells and mucin was demonstrated in vitro.

CRL 581 Differentially Modulates TLR3-Triggered Antiviral Innate Immune Response in Intestinal Epithelial Cells and Macrophages.

subsp. CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture subsp.

Characterization of UCO-SMC3 as a Potential Probiotic for the Skin: Its Beneficial Role in the Pathogenesis of Acne Vulgaris.

Previously, we isolated lactic acid bacteria from the slime of the garden snail Müller and selected UCO-SMC3 because of its ability to inhibit in vitro the growth of the skin-associated pathogen . The present study aimed to characterize the antimicrobial and immunomodulatory properties of UCO-SMC3 and to demonstrate its beneficial effect in the treatment of acne vulgaris. Our in vitro studies showed that the UCO-SMC3 strain resists adverse gastrointestinal conditions, inhibits the growth of clinical isolates of , and reduces the adhesion of the pathogen to keratinocytes.

Immunobiotic Feed Developed with subsp. TUA4408L and the Soymilk By-Product Okara Improves Health and Growth Performance in Pigs.

subsp. TUA4408L is able to differentially modulate the innate immune response of porcine intestinal epithelial cells triggered by TLR4 activation. This strain also has a remarkable ability to grow on plant substrates.

Immunomodulatory Properties of a γ-Aminobutyric Acid-Enriched Strawberry Juice Produced by CRL 2013.

Gamma-aminobutyric acid (GABA) plays a key role in mammals as the major inhibitory neurotransmitter of the central nervous system. Although GABA may not be able to cross the human blood-brain barrier, it was approved as a food ingredient because of its benefits to the host after oral administration including anti-hypertensive, anti-depressant and anti-inflammatory activities. Considering the current trend toward the development of new functional and natural products and that microbial fermentation is one of the most promising methods to produce this non-protein amino acid, the production of GABA through fermentation of strawberry and blueberry juices by the efficient GABA producer strain, (formerly known as ) CRL 2013, was evaluated.

Metabolic shift in the production of corrinoid compounds by Lactobacillus coryniformis in the absence of purines.

Lactobacillus coryniformis CRL 1001 and L. reuteri CRL 1098 have the complete genes necessary to synthesize pseudo-cobalamin as final product in a vitamin B free commercial medium. Unlike vitaminB (the most biologically active form), the pseudo-cobalamin contains adenine instead of 5,6-dimethlbenzimidazole (DMB) in the Coα-ligand.

YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus.

The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrueckii subsp.