Large-scale identification of proteins involved in the development of a sexually dimorphic behavior.

Sexually dimorphic behaviors develop under the influence of sex steroids, which induce reversible changes in the underlying neural network of the brain. However, little is known about the proteins that mediate these activational effects of sex steroids. Here, we used a proteomics approach for large-scale identification of proteins involved in the development of a sexually dimorphic behavior, the electric organ discharge of brown ghost knifefish, Apteronotus leptorhynchus.

Numerical chromosome variation and mitotic segregation defects in the adult brain of teleost fish.

Teleost fish are distinguished by their enormous potential for the generation of new cells in both the intact and the injured adult brain. Here, we present evidence that these cells are a genetic mosaic caused by somatic genomic alteration. Metaphase chromosome spreads from whole brains of the teleost Apteronotus leptorhynchus revealed an euploid complement of 22 chromosomes in only 22% of the cells examined.

New neurons for the injured brain: mechanisms of neuronal regeneration in adult teleost fish.

In contrast to mammals, teleost fish exhibit an enormous potential to continuously produce new neurons in many areas of the adult brain, and to regenerate neural tissue after brain injury. The regenerative capability of the teleost fish brain is based upon a series of well-orchestrated individual processes, including: elimination of damaged cells by apoptosis, removal of cellular debris by the action of microglia/macrophages, proliferation of endogenous neural precursor cells, radial glia-mediated migration of their progeny to the site of the lesion, neuronal differentiation, promotion of cellular survival, and integration of the new neurons into existing neural circuits. Combination of a well-defined cerebellar lesion paradigm with differential proteome analysis has demonstrated that identification of the multitude of proteins mediating the regenerative potential of the adult fish brain is feasible in the foreseeable future.

Upregulation of calbindin-D28k expression during regeneration in the adult fish cerebellum.

In contrast to mammals, fish are distinguished by their enormous potential for brain repair after injuries. This phenomenon has been well studied after application of stab-wound lesions to the corpus cerebelli, a cerebellar subdivision, in the teleost fish Apteronotus leptorhynchus. By combining this lesion paradigm with immunohistochemical staining, we examined the potential role of the calcium-binding protein calbindin-D(28k) in the process of regeneration.

Proteome analysis identifies novel protein candidates involved in regeneration of the cerebellum of teleost fish.

In contrast to mammals, adult teleost fish exhibit an enormous potential to regenerate neuronal tissue after injuries to the CNS. By combining a well-defined cerebellar lesion paradigm with differential proteome analysis at a post-lesion survival time of 3 days, we screened for protein candidates involved in repair of the fish brain. Out of nearly 900 protein spots detected on 2-D gels, spot intensity was significantly increased at least twofold in 30 spots and decreased to at least half the intensity of control tissue in 23 spots.