Publications by authors named "Mariana X Navarro"
Article Synopsis
- Macrophages can behave in two main ways when activated: inflammatory (M1) or anti-inflammatory (M2), and tracking these behaviors is important for understanding immune function.
- Current methods for monitoring macrophage polarization are either static or not suitable for complex environments, so the researchers developed a new approach using genetically engineered luciferase reporters.
- Their method allows for long-term observation of macrophage types in both 2D and 3D formats with high resolution, paving the way for better monitoring of immune cells and other complex biological networks.
Article Synopsis
- Macrophages can be classified into two types based on activation: inflammatory (M1) and anti-inflammatory (M2), which are important for understanding immune function.
- Current methods for tracking these types are limited in their ability to provide real-time data, especially in complex 3D environments.
- This study introduces new bioluminescent technologies that allow for long-term monitoring of macrophage polarization using genetically engineered reporters, enhancing the ability to observe these cells in living samples and enabling more detailed analysis in various settings.
Caged luciferins enable rapid multicomponent bioluminescence imaging.
Photochem Photobiol
January 2024
Bioluminescence is a sensitive technique for imaging biological features over time. Historically, though, the modality has been challenging to employ for multiplexed tracking due to a lack of resolvable luciferase-luciferin pairs. Recent years have seen the development of numerous orthogonal probes for multi-parameter imaging.
View Article and Find Full Text PDFA compelling link is emerging between the posttranslational modification O-GlcNAc and protein aggregation. A prime example is α-synuclein, which forms toxic aggregates that are associated with neurodegeneration in Parkinson's and related diseases. α-Synuclein has been shown to be O-GlcNAcylated at nine different positions in in vivo proteomics experiments from mouse and human tissues.
View Article and Find Full Text PDF