Experimental design of a culture approach for corneal endothelial cells of New Zealand white rabbit.

The harvesting of corneal endothelial cells (CEC) has received special attention due to its potential as a therapy for corneal blindness. The main challenges are related to the culture media formulation, cellular density at the primary isolation, and the number of passages in which CEC can retain their functional characteristics. To alternate different media formulations to harvest CEC has an impact on the cellular yield and morphology.

Corneal perforation associated with isolated ocular lichen planus: a case report.

Introduction: To report a case of unilateral corneal perforation due to isolated ocular lichen planus.

Methods: Interventional case report. Informed consent by the patient was obtained to publish clinical images.