Publications by authors named "Mariana Duque Mello"
Article Synopsis
- The study compared two staining techniques, Diff-Quick and Spermac, to see how they affect the assessment of sperm morphology and influence infertility diagnoses.
- Results showed that while both methods clearly displayed sperm structures, Diff-Quick identified a higher percentage of normal spermatozoa compared to Spermac, which highlighted more midpiece abnormalities.
- The findings suggest that the choice of staining technique is crucial for accurate diagnoses of male infertility, indicating a need for further research on optimal methods.
Background: Leptin and its receptor are present in spermatozoa; however, the role of leptin in sperm function is still controversial. Our present study aimed at demonstrating the effect of cryopreservation on sperm DNA fragmentation (DNAf) and investigating the possible effects of sperm capacitation techniques and leptin incubation on frozen-thawed sperm DNAf and oxidative stress.
Methods: Samples of 45 normospermic men attending for infertility investigation at Vida Centro de Fertilidade, Rio de Janeiro, Brazil, were frozen and thawed with or without capacitation and leptin incubation prior to freezing.