A critical analysis of three quantitative methods of assessment of hepatic steatosis in liver biopsies.

The issue of adequately quantitatively evaluating hepatic steatosis is still unresolved. Therefore, we compared three methods of quantitative assessment. Two groups of mice (n = 10 each) were fed standard chow (10% fat, SC group) or a high-fat diet (60% fat, HF group) for 16 weeks, and hepatic triglyceride (HT) and liver tissue were then studied.

Modulation of cytokines, resistin, and distribution of adipose tissue in C57BL/6 mice by different high-fat diets.

Objective: To investigate whether changing the lipid source induces metabolic changes and/or modulates the adipose tissue distribution in mice fed with a high-fat (HF) diet.

Methods: C57BL/6 mice were subjected to a 10-wk control diet (10% fat) or an HF diet (60% fat) containing lard (HF-L), olive oil (HF-O), sunflower oil, or canola oil. Food intake and body weight were measured.

Adult cardiorenal benefits from postnatal fish oil supplement in rat offspring of low-protein pregnancies.

We investigated the effect of fish oil (FO) treatment on cardiorenal structure of adult offspring from low-protein pregnancies. Three month old offspring were assigned to eight groups (four male groups and four female groups, n=8 each) (NP=normal-protein diet, LP=low-protein diet): NP, LP, NP plus FO, and LP plus FO. Left ventricle and kidney were analyzed with light microscopy and stereology.