Diagnostic accuracy of interleukin-6 levels in peritoneal fluid for detection of endometriosis.

Purpose: To determine, with extended receiver operating characteristic (ROC) curve analysis, the diagnostic value of cytokines showing significantly different peritoneal concentrations between women with and without endometriosis.

Methods: Multiplex cytokine concentration measurement of IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ levels in peritoneal fluid of women with minimal to mild (n = 10) and moderate to severe (n = 26) endometriosis, and 42 controls.

Results: Only IL-6 and IL-10 concentrations were significantly higher in endometriosis patients than in controls.

Occurrence of Mycoplasma genitalium in fertile and infertile women.

Objective: To determine the frequency of occurrence of Mycoplasma genitalium in the reproductive organs of infertile women in comparison with a control group of healthy, fertile women.

Design: Prospective study.

Setting: Gynecology Clinic at the 2nd Department of Gynecology and Obstetrics of the Wroclaw Medical University, Poland.

Serum and peritoneal evaluation of vitamin D-binding protein in women with endometriosis.

Introduction: Vitamin D-binding protein (also called DBP or Gc-globulin) is recognized as a multifunctional protein involved in the action scavenger system, the transport of vitamin D sterols, and the modulation of immune and inflammatory responses. This study evaluated total serum and peritoneal concentrations of vitamin D-binding protein in women with endometriosis, known as an inflammation-associated disease.

Materials/methods: The total concentration of DBP was measured with an enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody raised in a goat immunized with human DBP.

Flow cytometric evaluation of intracellular cytokine synthesis in peripheral mononuclear cells of women with endometriosis.

Systemic changes related to cytokine expression levels in women with endometriosis remain a subject of controversy. There are many studies concerning this topic showing differential serum cytokine levels; however, there are limited data presenting cytokine expression at the single-cell level. This study focused on this question by measuring intracellular cytokine staining of activated peripheral CD3+ and CD14+ cells from women with endometriosis (investigative group) compared with those with uterine leiomyoma (control group).

[Frequency of detection of Ureaplasma urealyticum and Mycoplasma hominis in cervical canal and the Douglas pouch of infertile and fertile women].

The group of organisms commonly referred to as genital mycoplasmas comprise species most often found in genitourinary tract of sexually active adults as common commensal inhabitants, or pathogens which can possibly cause many different pathologies like: non-gonococcal urethritis, bacterial vaginosis, cervicitis, endometritis or pelvic inflammatory disease. The problem of their morbidity and the possible influence they have on human fertility is still not clear. The aim of this study was to find out whether two investigated species- Ureaplasma urealyticum and Mycoplasma hominis can be detect more often in a group of infertile women.

Differential flow cytometric detection of intracellular cytokines in peripheral and peritoneal mononuclear cells of women with endometriosis.

Objective: The pathogenesis of endometriosis is related to functional changes in CD3+ and CD14+ cells observed both at the local and systemic level. Here we investigated whether, and if so, how the body compartment influences cytokine expression in stimulated peritoneal and peripheral CD3+ and CD14+ cells of women with endometriosis.

Study Design: Isolated peripheral blood (PB) and peritoneal fluid (PF) mononuclear cells from women with endometriosis were cultured under non-adherent conditions and stimulated with PMA and ionomycin for 6h to induce intracellular cytokine synthesis of TNF-alpha, IFN-gamma, and IL-8 by CD3+ cells or with LPS for 9h to produce TNF-alpha, IL-6, IL-10, MCP-1, and IL-8 by CD14+ cells.

Bicornuate rudimentary uterine horns with functioning endometrium and complete cervical-vaginal agenesis coexisting with ovarian endometriosis: a case report.

Objective: To report a patient with bicornuate rudimentary uterine horns with functioning endometrium and complete cervical-vaginal agenesis coexisting with ovarian endometriosis.

Design: Case report.

Setting: University hospital.

[The presence of Mycoplasma hominis and Ureaplasma urealyticum in the cervical canal of uterus].

Objectives: Mycoplasma hominis and Ureaplasma species are common commensal inhabitants of the lower genitourinary tract in adolescents and adults who are sexually active. A lot of authors points out that these microorganisms can play an important role in pathology of genital tract like pelvic inflammatory disease, sterility or non-gonococcal urethritis.

Materials And Methods: In the study samples from cervical canal of the uterus were obtained from 222 women.

The coexistence of ERBB2, INT2, and CMYC oncogene amplifications and PTEN gene mutations in endometrial carcinoma.

Purpose: To evaluate the frequency of ERBB2, INT2 and CMYC oncogene amplifications and their coexistence with PTEN gene mutations in endometrial carcinomas.

Methods: In 54 endometrial carcinomas amplification of ERBB2, INT2 and CMYC was determined using differential polymerase chain reaction (dPCR), and mutations in all exons of PTEN were investigated by PCR-SSCP and direct sequencing methods. Results were correlated with clinicopathological features of tumors.

[Own experiences in salvage chemotherapy of ovarian cancer--a preliminary report].

Objective: To compare the efficacy and side effects of the ovarian cancer salvage treatment.

Materials And Methods: 28 patients from 2nd Department of Gynecology of Wrocław Medical University, treated for recurrent ovarian cancer in stage III and IV were analyzed. First line treatment after complete debulking operation was based on platinum analogue (CAP).

[Ovarian cancer in a pregnant woman and limited treatment strategy--a case report].

We present the case of 31-year-old woman in 13th week of pregnancy with the diagnosis of the ovarian cancer (cystadenocarcinoma papillare serosum G1) in the FIGO stage Ia. Taking into consideration this data and the patient's wish of preserving the pregnancy and the childhood bearing possibility in the future we performed only left salpingooophorectomy. Postoperative and further pregnancy course was uncomplicated.

[Course of pregnancy in a patient with systemic lupus erythematosus--case report].

Authors describe a successful pregnancy outcome after prednisone and aspirin therapy in 19-year-old woman with systemic lupus erythematosus with the history of recurrent urinary tract infections, pericarditis and encephalitis.

[Evaluation of expression and correlation between P53, BCL-2 and BAX proteins in ovarian neoplasms].

Objectives: Interactions between BCL-2 and other proteins regulating programmed cells death in ovarian carcinomas are poorly understood.

Design: The evaluation of expression of P53, BCL-2 and BAX proteins in ovarian carcinomas. The associations between oncoproteins studied and the histological structure, grade of differentiation and stage of disease were also analysed.

Assessment of the quality and frequency of mutations occurrence in PTEN gene in endometrial carcinomas and hyperplasias.

The quality and frequency of mutations in PTEN gene were assessed in 59 carcinomas and 6 hyperplasias of the endometrium in women. Screening for mutations was done in all exons of PTEN gene by the PCR-SSCP analysis and DNA sequencing. Results were correlated with histological status and clinical features of endometrial carcinomas.