Cytotoxic effect of kernel extract on HCT116 and A2058 cancer cell lines.

While the pharmacology of leaf extract has been studied extensively, little is known about the pharmacological potential of seeds, although they contain similar active ingredients that are responsible for the therapeutic effects of the leaf extract. In this study we used 70%-methanol kernel extract, quantified its bioactive constituents and tested their cytotoxic effect on two cancer cell lines, A2058 and HCT116, and the non-tumor cell line McCoy-Plovdiv. We studied the biological effect of the extract by real-time analysis in the xCELLigence system, WST-1 assay and LIVE/DEAD viability assay.

Investigation Of Chromium-Cobalt Coated Scaffolds On Cell Cultures In Vitro.

Introduction: The use of diverse materials for medical purposes is continuously expanding. The modification of materials which are routinely applied in medical practice as well as the development and introduction of new materials require studies on their biological activity. The first steps in this process are the preliminary short-term screening tests for cytotoxicity and biocompatibility performed on cell cultures.

Micromeritic procedures in assessing antinuclear antibody patterns in immunofluorescent assay.

Aim: The aim of this study was to introduce a micromeritic procedure (a statistical approach for small objects) in indirect immunofluorescence assay (IFA) to find objective quantitative parameters of antinuclear antibody (ANA) patterns which could support a diagnosis of auto-immune diseases.

Materials And Methods: Sera of patients with systemic autoimmune diseases, McCoy-Plovdiv serum-free cell line, goat anti-human immunoglobulin-G FITC-conjugate, fluorescent microscope, computer-assisted digital image processing, analysis using a micromeritic procedure, ANOVA.

Results: Three ANA fluorescent patterns (homogeneous, rim and speckled) were analyzed by the micromeritic procedure.

A comparative study of immunological methods for determination of serum antinuclear antibodies.

Introduction: Several immunological methods are used to determine the serum antinuclear antibodies (ANA). Indirect immunofluorescence assay (IFA) with tissue slices or HEp-2 cells is the standard technique considered the gold standard for their screening. Serum-free McCoy-Plovdiv cell line may also be used as substrate for IFA.

Guidelines for cultivation and preservation of the serum-free cell line McCoy-Plovdiv.

In this paper, we offer detailed information concerning manipulations with the novel serum-free cell line McCoy-Plovdiv. Guidelines for procedures as trypsinization of the monolayer, subculturing, as well as freezing and thawing conditions are proposed. Our results give us grounds to assume that this is a cell line entirely serum-independent at any step of the process of culturing and preservation.

Indirect immunofluorescent assay for antinuclear antibodies on McCoy-Plovdiv serum-free cell line substrate.

Indirect immunofluorescent assay for antinuclear antibodies (IIFA-ANA) on HEp-2 cell substrate is a widely used test for diagnosis of connective tissue diseases. Recently, serum-free fibroblast cell line McCoy-Plovdiv has been developed to provide an alternative substrate. The aim of the present study was to evaluate the diagnostic performance of IIFA-ANA on McCoy-Plovdiv cell substrate for systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and compare it with that of the standard HEp-2 cell substrate.