Quantification of MRP8 in immunohistologic sections of peri-implant soft tissue: Development of a novel automated computer analysis method and of its validation procedure.

Background: As manual cell counting lacks objectivity in the assessment of positive marker cells in immunohistologic sections, there has been a shift to automated computer analysis solutions. However, quantifying inflammation around dental implants is still often done by manual cell counting.

Method: With mucosal sections stained against MRP8 harvested around dental implants, we developed an automated method (AM) to identify positive marker cells.