Publications by authors named "Mariam Huda"
Determination of Anaphase Duration by Time-Lapse Microscopy in Budding Yeast.
Methods Mol Biol
November 2024
Article Synopsis
- Time-lapse microscopy is an essential method for studying cell cycle dynamics, particularly to observe the duration of anaphase in budding yeast cells.
- The chapter provides a detailed guide on using this technique, covering everything from preparing samples and setting up the microscope to analyzing the data.
- By focusing on the mitotic spindle, the methodology helps researchers understand the kinetics of mitotic exit in cell division.
Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling lipid that plays crucial roles in various cellular processes, including endolysosomal system structure/function, stress response, and cell cycle regulation. PI(3,5)P2 synthesis increases in response to environmental stimuli, yet its behavior in cycling cells under basal conditions remains elusive. Here, we analyzed spatiotemporal changes in PI(3,5)P2 levels during the cell cycle of S.
View Article and Find Full Text PDFCoordination of mitotic exit with chromosome segregation is key for successful mitosis. Mitotic exit in budding yeast is executed by the mitotic exit network (MEN), which is negatively regulated by the spindle position checkpoint (SPOC). SPOC kinase Kin4 is crucial for SPOC activation in response to spindle positioning defects.
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