An Initial Proteomic Analysis of Biogas-Related Metabolism of Consortia in Sediments from the Santiago River, México.

In this paper, sediments from the Santiago River were characterized to look for an alternative source of inoculum for biogas production. A proteomic analysis of methane-processing archaea present in these sediments was carried out. The superkingdom of archaea is responsible for methane production and methane assimilation in the environment.

Effects of Ornamental Plant Density and Mineral/Plastic Media on the Removal of Domestic Wastewater Pollutants by Home Wetlands Technology.

Wastewater treatment (WWT) is a priority around the world; conventional treatments are not widely used in rural areas owing to the high operating and maintenance costs. In Mexico, for instance, only 40% of wastewater is treated. One sustainable option for WWT is through the use of constructed wetlands (CWs) technology, which may remove pollutants using cells filled with porous material and vegetation that works as a natural filter.

Caspase-3 Activation Correlates With the Initial Mitochondrial Membrane Depolarization in Neonatal Cerebellar Granule Neurons.

In this study we evaluated the effect of the reduction in the endoplasmic reticulum calcium concentration ([Ca]), changes in the cytoplasmic calcium concentration ([Ca]), alteration of the mitochondrial membrane potential, and the ER stress in the activation of caspase-3 in neonatal cerebellar granule cells (CGN). The cells were loaded with Fura-2 to detect changes in the [Ca] and with Mag-fluo-4 to measure variations in the [Ca] or with TMRE to follow modifications in the mitochondrial membrane potential in response to five different inducers of CGN cell death. These inducers were staurosporine, thapsigargin, tunicamycin, nifedipine and plasma membrane repolarization by switching culture medium from 25 mM KCl (K25) to 5 mM KCl (K5).

The Trans Golgi Region is a Labile Intracellular Ca Store Sensitive to Emetine.

The Golgi apparatus (GA) is a bona fide Ca store; however, there is a lack of GA-specific Ca mobilizing agents. Here, we report that emetine specifically releases Ca from GA in HeLa and HL-1 atrial myocytes. Additionally, it has become evident that the trans-Golgi is a labile Ca store that requires a continuous source of Ca from either the external milieu or from the ER, to enable it to produce a detectable transient increase in cytosolic Ca.

Differential expression and signaling of the human histamine H receptor isoforms of 445 and 365 amino acids expressed in human neuroblastoma SH-SY5Y cells.

In stably-transfected human neuroblastoma SH-SY5Y cells, we have compared the effect of activating two isoforms of 445 and 365 amino acids of the human histamine H receptor (hHR and hHR) on [S]-GTPγS binding, forskolin-induced cAMP formation, depolarization-induced increase in the intracellular concentration of Ca ions ([Ca]i) and depolarization-evoked [H]-dopamine release. Maximal specific binding (B) of [H]-N-methyl-histamine to cell membranes was 953 ± 204 and 555 ± 140 fmol/mg protein for SH-SY5Y-hHR and SH-SY5Y-hHR cells, respectively, with similar dissociation constants (K, 0.86 nM and 0.

Histamine H receptor activation stimulates calcium mobilization in a subpopulation of rat striatal neurons in primary culture, but not in synaptosomes.

The histamine H receptor (HR) is abundantly expressed in the Central Nervous System where it regulates several functions pre and postsynaptically. HRs couple to Gα proteins and trigger or modulate several intracellular signaling pathways, including the cAMP/PKA pathway and the opening of N- and P/Q-type voltage-gated Ca channels. In transfected cells, activation of the human HR of 445 amino acids (hHR) results in phospholipase C (PLC) stimulation and release of Ca from intracellular stores.

Acidic intracellular Ca(2+) stores and caveolae in Ca(2+) signaling and diabetes.

Acidic Ca(2+) stores, particularly lysosomes, are newly discovered players in the well-orchestrated arena of Ca(2+) signaling and we are at the verge of understanding how lysosomes accumulate Ca(2+) and how they release it in response to different chemical, such as NAADP, and physical signals. Additionally, it is now clear that lysosomes play a key role in autophagy, a process that allows cells to recycle components or to eliminate damaged structures to ensure cellular well-being. Moreover, lysosomes are being unraveled as hubs that coordinate both anabolism via insulin signaling and catabolism via AMPK.