Application of 16S rRNA next generation sequencing in ticks in Greece.

Tick-borne bacteria pose a significant threat to human and veterinary public health. Greece is a Mediterranean country with rich tick fauna and the most commonly detected tick-borne bacterial pathogens are members of the and species. The variable V2-V4 and V6-V9 regions of 16S rRNA gene of seven ticks belonging to four genera representative in Greece () were analysed using multiple primer pairs by next generation sequencing (NGS).