Sequencing and chromosomal localization of Fabp6 and an intronless Fabp6 segment in the rat.

The fatty acid binding protein 6 gene (Fabp6) codes for ileal lipid binding protein. After sequencing of rat Fabp6, the gene was localized in a radiation hybrid (RH) map on chromosome 10. An intronless Fabp6 segment was found in four related rat inbred strains (SHR; SHRSP; WKY; and OKA), but not in 62 other rat inbred strains.

Cholesterol and copper in the liver of rabbit inbred strains with differences in dietary cholesterol response.

In order to investigate whether cholesterol intake influences the hepatic copper content of rabbits, we compared the hepatic copper content of two rabbit inbred strains after feeding the animals a control or a cholesterol-rich diet. One strain was not reactive to dietary cholesterol (IIIVO/JU), whereas the other strain was reactive to dietary cholesterol (AX/JU). The coefficient of inbreeding (F) >0.

Genetic map of AFLP markers in the rat (Rattus norvegicus) derived from the H x B/Ipcv and B x H/Cub sets of recombinant inbred strains.

The amplified fragment length polymorphism (AFLP) technique has been used to enhance marker density in a large set of recombinant inbred strains (H x B and B x H) derived from a spontaneously hypertensive rat (SHR/OlaIpcv) and a Brown-Norway (BN.lx/Cub) inbred strain. Thirteen different primer combinations were tested and a total of 191 polymorphic bands were detected.

Quantitative trait loci influencing blood and liver cholesterol concentration in rats.

Objective: The LEW/OlaHsd and BC/CpbU rat inbred strains differ markedly in blood and hepatic cholesterol levels before and after a cholesterol-rich diet. To define loci controlling these traits and related phenotypes, an F2 population derived from these strains was genetically analyzed.

Methods And Results: For each of the 192 F2 animals, phenotypes were determined, and genomic DNA was screened for polymorphic microsatellite markers.