Comparative Studies for Cryopreservation of Shoot Tips by Droplet-Vitrification.

The objective of this work was to assess the suitability of the Droplet-vitrification protocol previously developed with shoot tips for the cryopreservation of six species. Shoot tips were precultured for 1 day on a medium with 0.3 M sucrose in the dark, loaded in a solution with 1.

Assessment of Vegetative Growth and Genetic Integrity of Regenerants after Cryopreservation.

Jacks. ex Andrews is the vanilla species with the most commercial and greatest economic importance. It has been used as a case study in different cryopreservation studies that involve three vitrification-based approaches: droplet-vitrification (D-V), V-cryoplate (V-Cp) and D-cryoplate (D-Cp).

Indirect Somatic Embryogenesis and Cryopreservation of Weber Cultivar 'Chato'.

Unlabelled: Weber cultivar 'Chato' represents an important genetic supply of wild severely in decline populations of 'Chato' for breeding and transformation programs. In this work, the indirect somatic embryogenesis and cryopreservation of Somatic Embryos (SEs) were investigated using the 'Chato' cultivar as a study case.

Methods: Embryogenic calli were induced by the cultivation of 1 cm of young leaves from in vitro plants on MS semisolid medium supplemented with 24.

Osmo- and cryotherapy of sugarcane ( spp. L.) shoot-tips infected with sugarcane mosaic virus (SCMV).

In this work, we investigated the effect of different osmoprotective treatments and of cryopreservation using a droplet-vitrification (D-V) protocol to eliminate sugarcane mosaic virus (SCMV) of shoot-tips excised from in vitro propagated infected plantlets. Shoot-tips of sugarcane ( spp. L.

Evaluation of in vitro shoot multiplication and ISSR marker based assessment of somaclonal variants at different subcultures of vanilla ( Jacks).

The effect of subculture cycles on somaclonal variation of using intersimple sequence repeat (ISSR) markers was analyzed. Nodal segments of 2 cm in length were established in vitro and multiplied by 10 subculture cycles in Murashige and Skoog (MS) medium supplemented with 8.86 μM BAP (benzylaminopurine).

Geneticallly enginereed trehalose accumulation improves cryopreservation tolerance of chrysanthemum (Dendranthema grandiflorum kitam.) shoot-tips.

Shoot-tips isolated from two transgenic lines of chrysanthemum (Dendranthema grandiflorum Kitam.) var. Indianapolis in vitro plantlets with induced capacity to biosynthesize trehalose, and from a non-transformed line, were subjected to cryopreservation using a vitrification procedure.

Cryopreservation of plant germplasm using the encapsulation-dehydration technique: review and case study on sugarcane.

Encapsulation-dehydration is a cryopreservation technique based on the technology developed for producing synthetic seeds, i.e. the encapsulation of explants in calcium alginate beads.