Molecular Response of Pulp Fibroblasts after Stimulation with Pulp Capping Materials.

This in vitro study evaluated cell viability and metabolism, nitric oxide release and production of two chemokines and one cytokine by cultured human dental pulp fibroblasts (HDPF) in contact with two glass ionomer cements (Ketac Molar-KM and Vitrebond-VB), Single Bond (SB) and calcium hydroxide (Dycal-DY). Cultures of HDPF were established by means of an explant technique. The specimens were prepared under sterile conditions and in disks measuring 5 mm x 2 mm obtained from a prefabricated mold and placed on a permeable membrane to avoid direct contact with the cells.

Management of dental caries guided by the ICDAS-LAA: a 28-month follow-up.

Dental caries is a significant oral health issue and public health concern. Currently conceptualized as a dynamic and multifactorial process, caries is a continuum of disease stages, during which tooth damage may be reversed or controlled, depending on lesion progression. The International Caries Detection and Assessment System, when used in conjunction with the Lesion Activity Assessment (ICDAS-LAA), reflects the dynamic caries process and allows the classification of cavitated lesions according to their severity and progression, which is essential to selecting the appropriate treatment.

Influence of depth of mesio-occlusodistal preparation and mechanical cycling on fracture resistance of restored premolars.

The purpose of this in vitro study was to evaluate the influence of depth (3.0 vs 5.0 mm) of mesio-occlusodistal (MOD) cavity preparation and mechanical cycling on fracture resistance of maxillary premolars restored with resin-based composite (RBC).

Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System.

This study evaluated in vitro cell viability and metabolism, nitric oxide release and production of chemokines by cultured human dental pulp fibroblasts (DPF) under contact with HEMA and Single Bond. Cultures of DPF were established by means of an explant technique. Once plated, cells were kept under contact with increasing concentrations of HEMA (10, 100 and 1000 nM) or Single Bond (SB) [10-fold serially diluted in culture medium (10-4, 10-3 and 10-2 v/v)] and also with polymerized SB components.

Influence of Erosive and Abrasive Cycling on Bonding of Different Adhesive Systems to Enamel: An In situ Study.

This study evaluated the impact of orange juice on the bond strength (BS) of dentin bonding systems (DBSs) to enamel surface after simulation with an in situ/ ex vivo erosive cycling. One hundred and ninety two bovine enamel fragments (4x4x2mm) were obtained and randomized regarding superficial microhardness and distributed to palatal devices for 8 volunteers, in three phases (one for each DBS), containing 8 blocks, which were, allocated in 4 pairs. Daily, these pairs were subjected extraorally to the following conditions: CONT- neither erosive nor abrasive challenge; ERO- erosive challenge only; ABR- abrasive challenge only and ERO + ABR- with erosive and abrasive challenges.

Water interaction and bond strength to dentin of dye-labelled adhesive as a function of the addition of rhodamine B.

Objective: This study investigated the effect of the fluorescent dye rhodamine B (RB) for interfacial micromorphology analysis of dental composite restorations on water sorption/solubility (WS/WSL) and microtensile bond strength to dentin (µTBS) of a 3-step total etch and a 2-step self-etch adhesive system.

Material And Methods: The adhesives Adper Scotchbond Multi-Purpose (MP) and Clearfil SE Bond (SE) were mixed with 0.1 mg/mL of RB.

Effect of resin-modified glass-ionomer cement lining and composite layering technique on the adhesive interface of lateral wall.

Unlabelled: Interface integrity can be maintained by setting the composite in a layering technique and using liners.

Objective: The aim of this in vitro study was to verify the effect of resin-modified glass-ionomer cement (RMGIC) lining and composite layering technique on the bond strength of the dentin/resin adhesive interface of lateral walls of occlusal restorations.

Material And Methods: Occlusal cavities were prepared in 52 extracted sound human molars, randomly assigned into 4 groups: Group 2H (control) - no lining + two horizontal layers; Group 4O: no lining + four oblique layers; Group V-2H: RMGIC lining (Vitrebond) + two horizontal layers; and Group V-4O: RMGIC lining (Vitrebond) + four oblique layers.

Bleaching technique effect on dentin permeability.

Purpose: To evaluate the effect of bleaching techniques on dentin permeability. The hypothesis was that the bleaching agent associated or not to etching and/or energy source would be able to increase dentin permeability (conductance).

Methods: Fifty 1 mm-thick disks of mid-coronal dentin were obtained from human third molars, which were mounted in a filtration chamber, allowing exposure of a standardized area of 0.

Push-out bond strength and SEM evaluation of a new bonding approach into the root canal.

Objective: This study evaluated the performance of different adhesive systems in fiber post placement aiming to clarify the influence of different hydrophobic experimental blend adhesives, and of one commercially available adhesive on the frictional retention during a luting procedure.

Material And Methods: One luting agent (70 Wt% BisGMA, 28.5% TEGDMA; 1.

Prevalence of enamel defects in permanent teeth of patients with complete cleft lip and palate.

Objective : To evaluate the prevalence, types, location, and characteristics of enamel defects in anterior permanent teeth of patients with complete unilateral and bilateral cleft lip and palate, as well as the relation with the cleft. Setting : Hospital for Rehabilitation of Craniofacial Anomalies, Bauru, São Paulo, Brazil. Participants : Eighty patients of both genders, 12 years and older, with unilateral or bilateral cleft lip and palate.

Microhardness and chemical analysis of high-viscous glass-ionomer cement after 10 years of clinical service as ART restorations.

Objectives: The aim of this study was to evaluate the Knoop microhardness (KHN) and chemical composition of high-viscous glass-ionomer cement (HVGIC) after 10 years of clinical service.

Methods: Six HVGIC samples were cut from 10-year ART restorations. The sections were embedded in acrylic moulds with their longitudinal profile exposed.

Effect of bleaching gels on surface roughness of nanofilled composite resins.

Objectives: The aim of this study was to investigate the superficial texture of composite restorations after different bleaching protocols.

Methods: Filtek Supreme (S), Filtek Z350 (F), and Grandio (G) were compared to Opallis (O) and Filtek Z250 (Z) (control microhybrid composites) and to bovine enamel using three different bleaching agents: 35% hydrogen peroxide Whiteness HP (WHP), 35% Whiteness HP MAXX (WMAXX) and 16% carbamide peroxide Whiteness Standard (WS). Six specimens from each composite were treated using each bleaching agent, according to the manufacturers' instructions.

Short-term in situ/ex vivo study of the anticariogenic potential of a resin-modified glass-ionomer cement associated with adhesive systems.

Objective: As resin-modified glass-ionomer cement (RMGIC) is an adhesive material, its association to dentin bonding agents (DBAs) was previously proposed. This study investigated the adjunctive behavior of an RMGIC with etch-and-rinse bonding systems under in situ/ex vivo cariogenic challenge.

Method And Materials: Bovine enamel blocks (3 3 3 3 2 mm) were randomly assigned to group VP, Vitremer + its own primer (3M ESPE); group VSB, Vitremer + Single Bond (3M ESPE); and group VPB, Vitremer + Prime and Bond 2.

Cytotoxicity and biocompatibility of direct and indirect pulp capping materials.

There are several studies about the cytotoxic effects of dental materials in contact with the pulp tissue, such as calcium hydroxide (CH), adhesive systems, resin composite and glass ionomer cements. The aim of this review article was to summarize and discuss the cytotoxicity and biocompatibility of materials used for protection of the dentin-pulp complex, some components of resin composites and adhesive systems when placed in direct or indirect contact with the pulp tissue. A large number of dental materials present cytotoxic effects when applied close or directly to the pulp, and the only material that seems to stimulate early pulp repair and dentin hard tissue barrier formation is CH.

In vitro interactions between lactic acid solution and art glass-ionomer cements.

Objectives: Production of acids such as lactic acid contributes to establish a cariogenic environment that leads to dental substrate demineralization. Fluoride plays an important role in this case and, as fluoride-releasing materials, glass-ionomer cements are expected to contribute to minimize deleterious reactions. This study evaluated interactions of glass-ionomer cements used in atraumatic restorative treatment (ART-GICs) with an aqueous lactic acid solution, testing the null hypotheses that no changes occur in the pH of the solution or on the surface roughness and mass of the ART-GICs when exposed to lactic acid solution over a 6-week period.

Influence of differently oriented dentin surfaces and the regional variation of specimens on adhesive layer thickness and bond strength.

Statement Of The Problem: Adhesive systems can spread differently onto a substrate and, consequently, influence bonding.

Purpose: The purpose of this study was to evaluate the effect of differently oriented dentin surfaces and the regional variation of specimens on adhesive layer thickness and microtensile bond strength (MTBS).

Materials And Methods: Twenty-four molars were sectioned mesiodistally to expose flat buccal and lingual halves.

Effect of surface treatments on the spreading velocity of simplified adhesive systems.

Objectives: To determine the roughness of glass surfaces submitted to different treatments and to correlate it with the spreading velocity of two adhesive systems.

Materials And Methods: Glass slides were used as substrates to evaluate the spreading velocity of Single Bond and Prime & Bond NT adhesive systems. Six different surface treatments were compared: 1) no treatment; 2) silanization (SL); 3) sandblasting (SB); 4) SB + SL; 5) 10% hydrofluoric acid treatment (HF); 6) HF + SL.

Effect of the number of coats of simplified adhesive systems on microleakage of dentin-bordered composite restorations.

Aim: This study tested the null hypothesis that there is no difference on microleakage of dentin-bordered composite restorations using single or double coats of adhesive from one-bottle adhesive systems.

Methods And Materials: The enamel surface was removed from freshly extracted bovine teeth, and standardized Class V cavities (3 x 3 x 1.5 mm) were made at the cervical areas of buccal surfaces.

Influence of temperature on the spreading velocity of simplified-step adhesive systems.

Background: Flowability and viscosity vary for different adhesive systems owing to differences in their composition. These characteristics can be modified by environmental temperature.

Purpose: The purpose of this study was to determine the influence of temperature on the spreading (flow capacity) of simplified-step adhesive systems.

Effect of the number of thermocycles on microleakage of resin composite restorations.

Thermocycling simulates, in vitro, thermal changes that occur in the oral cavity. The aim of this study was to evaluate the influence of the number of cycles on microleakage. Class V cavities (1.

Effect of one-bottle adhesive systems on the fluoride release of a resin-modified glass ionomer.

A dhesive systems associated to resin-modified glass ionomer cements are employed for the achievement of a higher bond strength to dentin. Despite this benefit, other properties should not be damaged. This study aimed at evaluating the short-time fluoride release of a resin-modified glass ionomer cement coated with two one-bottle adhesive systems in a pH cycling system.