Characterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesis.

We characterized multiple knock-out mutants of the four Arabidopsis sucrose phosphate synthase (SPSA1, SPSA2, SPSB and SPSC) isoforms. Despite their reduced SPS activity, spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc mutants displayed wild type (WT) vegetative and reproductive morphology, and showed WT photosynthetic capacity and respiration. In contrast, growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants was reduced compared with WT plants.

[Economic factors related to the Millennium Development Goals: a literature review].

Objectives: To systematize all the information published on the status of and progress made toward the Health-related Millennium Development Goals (HMDGs), as well as to understand associations with certain economic factors and the potential for success.

Methods: A search was conducted for all scientific articles covering the Millennium Development Goals in general, published from 1 January 2000 to 31 August 2006, in the electronic databases of the EBSCO, CSA Illumina, Thomson Gale, SwetsWise, and BIREME. All original articles in English or Spanish that evaluated HMDG status, progress, and determinants were selected.

Policy determinants affecting the hunger millennium development goal.

The possible effect of Government Consumption (a component of Gross Domestic Product (GDP)) on attainment of the hunger Millennium Development Goal (MDG1) was analyzed by evaluating the effect of macroeconomic, social, demographic and policy variables on average undernourished population. Eighty-four developing countries with data available on undernourished population were included in an ecological study. Regression models were applied to explore possible determinants of Undernourished between 1990 and 2004 and consequent achievement of the projected 2004 MDG1.

Induction of hypothermic conditions associated with increased micronuclei formation in sigma-1 receptor knockout mice after administration of the antipsychotic compound E-5842.

The antipsychotic sigma-1 (sigma(1)) receptor ligand E-5842 has been shown to increase micronucleated polychromatic erythrocyte (MNPCE) frequency in mouse bone marrow secondary to compound-induced hypothermia. Interaction with sigma(1) receptor has been considered a plausible contributing factor for E-5842-induced hypothermia, raising concern for a possible class effect of sigma receptor ligands in the mouse micronucleus (MN) test. We assessed the potential of E-5842 (200 mg/kg, oral) to produce hypothermic conditions associated with increased micronuclei formation in sigma(1) receptor knockout (sigma(1)R-KO) and wild type (WT) mice.

Formation of micronucleated erythrocytes in mouse bone-marrow under conditions of hypothermia is not associated with stimulation of erythropoiesis.

Conditions of marked and long-lasting hypothermia have been shown to increase the formation of micronucleated polychromatic erythrocyte (MNPCE) in mouse bone-marrow. Stimulation of erythropoiesis as a consequence of anoxic conditions associated with decreased body temperature has been suggested as a possible mechanism for hypothermia-induced micronucleus formation. We examined whether chemically induced hypothermic conditions that produced increased MNPCE formation were associated with stimulation of erythropoiesis by measuring erythropoietin (EPO) concentrations in blood.

Evaluation of the genotoxic potential of three phenyltetrahydropyridinyl butylazole-derived sigma-receptor ligand drug candidates.

Three structurally related phenyltetrahydropyridinyl butylazole (PTHPB)-derived drug candidates with sigma receptor-binding properties were evaluated for genotoxic potential in the ICH standard battery of genetic toxicology assays. These comprised an Ames test, a mouse-lymphoma assay, and a mouse bone-marrow micronucleus test. The maximum test concentrations in the in vitro assays were determined by the solubility and/or the cytotoxicity of the compounds.

Childhood experiences of violence in perpetrators as a risk factor of intimate partner violence: a systematic review.

Background: Perpetrators' experiences of violence during childhood are considered a risk factor for intimate partner violence (IPV). The objective of this study is to systematically review the characteristics and quality of papers which analyse the association between being battered during childhood, witnessing marital violence as a child within the family of origin and having an absent or rejecting father and the occurrence of IPV.

Methods: Nine scientific databases were consulted (1960-2004).

Knowledge gaps in scientific literature on maternal mortality: a systematic review.

Issues related to maternal mortality have generated a lot of empirical and theoretical information. However, despite the amount of work published on the topic, maternal mortality continues to occur at high rates and solutions to the problem are still not clear. Scientific research on maternal mortality is focused mainly on clinical factors.

[Medical scientific Romania in the European context].

Unlabelled: A national and international publications bibliometric analysis was assessed to evaluate the scientific research situation about Romania and the relation with the European Parliament Health objectives for 2003-2008.

Material And Methods: A bibliographical search in MEDLINE between 1999 and 2003 was performed. An annual review was done and the articles were classified on seven activity branches.

Assessment of the genotoxic potential of the antipsychotic sigma receptor ligand E-5842.

The genotoxic potential of E-5842, a sigma ligand compound being developed as an antipsychotic drug, was evaluated by means of an extensive battery of in vitro and in vivo assays. Negative results were obtained in an Ames test (up to 5000 μg/plate), a mouse lymphoma assay (up to 535.1 μg/ml (-S9) and 891.

[Recent history of the news coverage of violence against women in Spain, 1997-2001].

Objectives: To explore press coverage of violence against women between 1997 and 2001, and to analyze the temporal development of murders due to this cause in Spain and the social context in which these media events take place.

Methods: Quantitative content analyses were performed of 865 news items on violence against women in the Spanish newspapers El País, ABC and El Mundo (1997-2001). Absolute and relative frequencies, and relative risk (RR), 95% confidence intervals (95% CI), were calculated.

Effect of democracy on health: ecological study.

Can political regimes be singled out as a factor affecting health? Rating countries by the extent of their freedom is a useful proxy for measuring the effects of democracy on health related variables

Induction of micronuclei in mouse bone-marrow erythrocytes in association with hypothermia after administration of the sigma receptor ligand E-5842.

Oral administration of E-5842, a new sigma1 receptor ligand being developed as an antipsychotic drug, to male mice at single doses of 50, 100, 200 and 400 mg/kg produced marked and sustained decreases in rectal temperature. Both the intensity and the duration of the hypothermic effect increased with dose. Maximum decreases from the mean pre-administration temperature (36.

[The gender approach in health-related newspaper articles].

Objectives: To use a gender approach to analyze the content of health-related articles affecting both sexes (cancer, heart attack and smoking) or mainly women (anorexia, domestic violence and abortion), published in newspapers in the 1990s. To provide recommendations for writing news with a gender-sensitive approach.

Material And Methods: Analysis of the above-mentioned topics in articles published in the national Spanish daily newspapers El País, ABC and El Mundo (1991-1999).

[Identifying barriers to food and nutrition policies in Colombia: a study using the Delphi method].

Objective: To identify the most important barriers to the development and progress of Colombia's National Food and Nutrition Plan (NFNP) (which was enacted in 1996), from the viewpoint of food and nutrition plan planners, implementers, and academicians.

Methods: The research was carried out in two stages. The first stage consisted of a pilot study in 1998 based on individual interviews with 35 key informants with knowledge of nutrition policies in Colombia, in order to identify possible groups of experts with experience in the development of the NFNP and also to confirm the relevance and feasibility of the research.

High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance.

Virus-induced gene silencing was used to assess the function of random Nicotiana benthamiana cDNAs in disease resistance. Out of 4992 cDNAs tested from a normalized library, there were 79 that suppressed a hypersensitive response (HR) associated with Pto-mediated resistance against Pseudomonas syringae. However, only six of these clones blocked the Pto-mediated suppression of P.

Differential expression and function of Arabidopsis thaliana NHX Na+/H+ antiporters in the salt stress response.

The Arabidopsis thaliana vacuolar Na+/H+ antiporter AtNHX1 is a salt tolerance determinant. Predicted amino acid sequence similarity, protein topology and the presence of functional domains conserved in AtNHX1 and prototypical mammalian NHE Na+/H+ exchangers led to the identification of five additional AtNHX genes (AtNHX2-6). The AtNHX1 and AtNHX2 mRNAs are the most prevalent transcripts among this family of genes in seedling shoots and roots.