AU-rich elements in the mRNA 3'-untranslated region of the rat receptor for advanced glycation end products and their relevance to mRNA stability.

Several putative polyadenylation sequences and an adenylate plus timidylate rich element (ARE) are present at the 3' end of the rat advanced glycation end products receptor (RAGE) gene. Two transcripts are generated by the use of alternative polyadenylation sequences, one containing the ARE sequence in its 3'-untranslated region (3'-UTR). Transfections of CHO-k1 or NRK cells with constructs expressing the 3'-UTRs of the transcripts fused to a green fluorescence protein mRNA show that the ARE sequence has a negative effect on protein expression correlating with a decrease in the amount of mRNA, as shown in CHO-k1 transfected cells.

Three-year clinical evaluation of In-Ceram Zirconia posterior FPDs.

Purpose: The purpose of this study was to evaluate the clinical performance of In-Ceram Zirconia posterior fixed partial dentures (FPD) after 3 years in service.

Materials And Methods: Eighteen In-Ceram Zirconia FPDs were fabricated in 16 patients. The FPDs were placed between January and April 1999.

Surgical treatment of late-onset post-traumatic partial seizures in a child.

Introduction: Although post-traumatic epilepsy accounts for a small number of epileptic patients, it should not be underestimated since it primarily affects children and young adults and can result in psychosocial disability and death.

Case Report: We present the case of a 14-year-old girl referred to us because of refractory partial seizures. The patient had experienced a head trauma at the age of 6 months requiring surgical treatment due to a large right fronto-temporo-parietal extradural hematoma.

Comparison of the marginal fit of Procera AllCeram crowns with two finish lines.

Purpose: The purpose of this study was to determine the influence of two finish line configurations on the marginal accuracy of Procera AllCeram crowns.

Materials And Methods: Twenty mechanized specimens of brass were fabricated for complete-coverage crowns. Two finish line designs were used: chamfer and rounded shoulder.

Comparing nutrient intake from food to the estimated average requirements shows middle- to upper-income pregnant women lack iron and possibly magnesium.

Objective: To determine whether nutrient intake from food alone was adequate across trimesters for middle- to upper-income pregnant women when compared with estimated average requirements (EAR), and to determine whether food intake exceeded the tolerable upper intake level (UL) for any nutrient.

Design: Observational study in which pregnant women completed 3-day diet records each month during their pregnancy. Records were analyzed for nutrient content, and usual intake distributions were determined.

Molecular and enzymatic analysis of ammonium assimilation in woody plants.

Ammonium is assimilated into amino acids through the sequential action of glutamine synthetase (GS) and glutamate synthase (GOGAT) enzymes. This metabolic pathway is driven by energy, reducing power and requires the net supply of 2-oxoglutarate that can be provided by the reaction catalysed by isocitrate dehydrogenase (IDH). Most studies on the biochemistry and molecular biology of N-assimilating enzymes have been carried out on annual plant species and the available information on woody models is far more limited.