Publications by authors named "Maria Sighicelli"

Indoor production of basil ( L.) is influenced by light spectrum, photosynthetic photon flux density (PPFD), and the photoperiod. To investigate the effects of different lighting on growth, chlorophyll content, and secondary metabolism, basil plants were grown from seedlings to fully expanded plants in microcosm devices under different light conditions: (a) white light at 250 and 380 μmol·m·s under 16/8 h light/dark and (b) white light at 380 μmol·m·s under 16/8 and 24/0 h light/dark.

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Indoor farming of basil ( L.) under artificial lighting to support year-round produce demand is an area of increasing interest. Literature data indicate that diverse light regimes differently affect downstream metabolic pathways which influence basil growth, development and metabolism.

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Once dispersed in water, microplastic (MP) particles are rapidly colonised by aquatic microbes, which can adhere and grow onto solid surfaces in the form of biofilms. This study provides new insights on microbial diversity and biofilm structure of plastisphere in lake waters. By combining Fourier Confocal Laser Scanning Microscopy (CLSM), Transform Infrared Spectroscopy (FT-IR) and high-throughput DNA sequencing, we investigated the microbial colonization patterns on floating MPs and, for the first time, the occurrence of eukaryotic core members and their possible relations with biofilm-forming bacterial taxa within the plastisphere of four different lakes.

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Understanding the spatial distribution patterns of microplastics (plastics < 5 mm) contributes to the assessment of sources and sinks of pollution thus providing information for the management of biota safety and overall ecosystem functionality. We chose a semi-closed study area, Lake Bracciano (Italy), to assess the environmental variability of contamination, focusing on the water compartment and the exposure of biota, specifically fish, by analysing the ingestion of microplastics. The focus of this study is to evaluate the concentration of microplastics in water (surface and column) across the lake and the ingestion of microplastics by two fish species of economic interest: Atherina boyeri and Coregonus lavaretus, inhabiting demersal and pelagic habitats respectively.

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This study investigated the biogeography, the presence and diversity of potentially harmful taxa harbored, and potential interactions between and within bacterial and eukaryotic domains of life on plastic debris in the Mediterranean. Using a combination of high-throughput DNA sequencing (HTS), Causal Network Analysis, and Scanning Electron Microscopy (SEM), we show regional differences and gradients in the Mediterranean microbial communities associated with marine litter, positive causal effects between microbes including between and within domains of life, and how these might impact the marine ecosystems surrounding them. Adjacent seas within the Mediterranean region showed a gradient in the microbial communities on plastic with non-overlapping endpoints (Adriatic and Ligurian Seas).

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Microplastic particles (MPs) contamination of aquatic environments has raised a growing concern in recent decades because of their numerous potential toxicological effects. Although fish are among the most studied aquatic organisms, reports on MPs ingestion in freshwater environments are still scarce. Thus, there is still much to study to understand the uptake mechanisms, their potential accumulation among the food webs and their ecotoxicological effects.

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In this study, 16S rRNA gene high throughput sequencing and Fluorescence In Situ Hybridization (FISH) combined with confocal laser scanning microscopy (CLSM) were used to assess for the first time biodiversity and structure of microplastic-associated biofilms (plastisphere) collected from Italian lentic ecosystems. The analysis revealed clear differences in microbial community composition among biofilms and corresponding planktonic populations indicating a selective adhesion on microplastics (MP). Although geographical variations in taxa composition were observed, a plastisphere core microbiome, composed by known biofilm formers found in freshwater ecosystems (e.

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Studies related to the evaluation of plastics in freshwaters have been increasing in recent years because approximately 80% of plastic items found in the sea are from inland waters. Despite the ecological relevance of these surveys, no information has been available until now about the hazard related to plastic mixtures in freshwaters. To fill this knowledge gap, we carried out a study aimed to assess the environmental risk associated with the "cocktail" of plastics and environmental pollutants adsorbed on their surface in one of the larger European freshwater basins.

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Using Fourier transform infrared spectroscopy (FT-IR) measurements and comparing the spectrum peaks (range 4000-600 cm) with reference spectra database and instrument libraries, we observed new evidence of the ingestion of microplastic particles analyzing the digestive tracts of Talitrus saltator. Specimens, sampled in central Italy, probably ingested the particles with natural detritus. Since worldwide many species of invertebrates and vertebrates (e.

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Plastic debris incidence in marine environment was already highlighted in the early 1970s. Over the last decade, microplastic pollution in the environment has received increasing attention and is now an emerging research area. Many studies have focused on quantifying microplastic abundance in the marine environment, while there are relatively few data on microplastic occurrence in freshwater environment.

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Characterization of beach debris is crucial to assess the strategy to answer questions such as recycling. With the aim to assess its use in a recycling scheme, in this note, we carried out a physical and chemical characterization of plastic litter from a pilot beach in Central Italy, using the FT-IR spectroscopy and thermoanalysis. Fourteen polymers, having mainly thermoplastic origin, were identified; among them, the most represented are polyethylene (41.

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Exposure of plants to high-heavy metals concentration inhibits multiple metabolic processes in plants and leads to an oxidative stress commonly referred as heavy metal ion toxicity. Chlorophyll a fluorescence has enhanced understanding of heavy metal ion action on the photosynthetic system. A rapid and non-invasive technique involving imaging of chlorophyll fluorescence is a useful tool for early detection of plant responses to heavy metal ion toxicity.

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In this article, a method to reveal the presence of Mg content inside the different parts of leaves of Hedera helix is presented. In fact a sample of a Hedera helix's leaf, commonly characterized by a green and a white side, is analyzed under X-ray radiation. The presence of two zones with different colors in the Hedera helix's leaf has not been explained.

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