Protein detection by nanopores equipped with aptamers.

Protein nanopores have been used as stochastic sensors for the detection of analytes that range from small molecules to proteins. In this approach, individual analyte molecules modulate the ionic current flowing through a single nanopore. Here, a new type of stochastic sensor based on an αHL pore modified with an aptamer is described.

One and two photon fluorescent complexes of rhenium and their technetium analogues.

The synthesis of rhenium and 99m-technetium complexes of dipyrazolemethanecarboxylates directly from the tetroxometallates is reported together with a 'one pot' synthesis of mixed ligand M(V) complexes. The rhenium complexes in all three oxidation states show strong fluorescence when the pyrazole groups have aromatic substituents with emission around 330 nm with both one and two photon excitation. The emission lifetimes of the complexes and free ligands differ considerably.

Syntheses and structures of pyrazolylmethane complexes of rhenium(III), (IV) and (V).

Reaction of [ReOCl3(PPh3)(2)] with HCpz(3) (pz = pyrazole) in dichloromethane leads to the formation of a new Re(iv) complex [ReCl3(HCpz3)]X (X=Cl, [ReO4]) with loss of the rhenium-oxo group. We also report a convenient, high-yield synthetic route to complexes of the type [ReOXn(L)](3-n)+ (X=Cl, Br, n = 2, 3) by the reaction of bis(pyrazolylmethane) and bis(pyrazolylacetate) ligands with [ReOCl3(PPh3)2]. Dinuclear complexes containing the O=Re-O-Re=O group were also isolated and structurally characterised.