Publications by authors named "Maria Petrovna Kruglova"
Simultaneous determination of cystine and other free aminothiols in blood plasma using capillary electrophoresis with pH-mediated stacking.
Electrophoresis
March 2024
We developed a method of sensitive capillary electrophoresis using UV detection for the determination of certain free aminothiols (reduced cysteinylglycine (rCysGly), cysteine (rCys), glutathione (rGln), and cystine (CysS) in human blood plasma. The reduced thiols were derivatized with N-ethylmaleimide. The plasma was purified from proteins via ultrafiltration.
View Article and Find Full Text PDFWe examined standard clinical and laboratory biochemical parameters, as well as the levels of aminothiols in the blood and urine (homocysteine (Hcy), cysteine (Cys), S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH)) via capillary electrophoresis in patients with CKD at stages II-V. Patient outcomes were assessed after five years. To complete forecasting, correlation and ROC analysis were performed.
View Article and Find Full Text PDFCoronary artery disease (CAD) and the coronary artery bypass graft (CABG) are associated with a decreased blood glutathione (bGSH) level. Since GSH metabolism is closely related to other aminothiols (homocysteine and cysteine) and glucose, the aim of this study was to reveal the associations of bGSH with glucose and plasma aminothiols in CAD patients (N = 35) before CABG and in the early postoperative period. Forty-three volunteers with no history of cardiovascular disease formed the control group.
View Article and Find Full Text PDFDetermination of glutathione in blood via capillary electrophoresis with pH-mediated stacking.
Electrophoresis
October 2022
Article Synopsis
- A new method using capillary electrophoresis has been developed to accurately measure reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG) in whole blood.
- The process includes stable sample preparations and a fast analysis time of only 8 minutes, with quantification limits for GSSG at 1.5 µM.
- Results from healthy volunteers show average levels of GSH, GSSG, and their ratio, indicating that this approach is suitable for clinical and laboratory applications.
Objective: S-Adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are indicators of global transmethylation and may play an important role as markers of severity of COVID-19.
Methods: The levels of plasma SAM and SAH were determined in patients admitted with COVID-19 ( = 56, mean age = 61). Lung injury was identified by computed tomography (CT) in accordance with the CT0-4 classification.
Association of Low Molecular Weight Plasma Aminothiols with the Severity of Coronavirus Disease 2019.
Oxid Med Cell Longev
October 2021
Article Synopsis
- Aminothiols, like glutathione (GSH) and cysteinylglycine (CG), might be important in understanding the severity of COVID-19, but their exact role hasn't been fully explored yet.
- A study of 59 COVID-19 patients found that lower total glutathione (tGSH) levels were linked to a higher risk of severe illness and more lung damage, while levels of reduced cysteinylglycine (rCG) were also associated with lung injury.
- The findings suggest that tGSH and rCG could serve as useful indicators for assessing COVID-19 severity, highlighting the need to potentially adjust glutathione levels as part of treatment.
Objective: Acute brain ischemia is accompanied by a disruption of low-molecular-weight aminothiols (LMWTs) homeostasis, such as homocysteine (Hcy), cysteine (Cys), and glutathione (GSH). We investigated the redox balance of LMWTs in blood plasma and its influence on ischemic stroke severity and the functional outcome in patients with an acute period.
Patients And Methods: A total of 177 patients were examined.
Objective: To determine whether urine S-adenosylmethionine (SAM) might be an indicator of chronic kidney disease (CKD).
Methods: We investigated urine levels of SAM and related metabolites (S-adenosylhomocysteine and homocysteine cysteine) in 62 patients (average age, 65.9 years) with CKD (stages II-V).
Determination of S-adenosylmethionine and S-adenosylhomocysteine in blood plasma by UPLC with fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci
August 2019
A validated approach to determine various methionine cycle metabolites (S-adenosylmethionine, S-adenosylhomocysteine, and methylthioadenosine) in human blood plasma is offered. The approach is based on solid-phase extraction (with grafted phenylboronic acid) and derivatization with chloroacetaldehyde followed by ultra-performance liquid chromatography with fluorescence detection. We used a 100 × 2.
View Article and Find Full Text PDFObjective: To evaluate the association of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in urine with chronic kidney disease (CKD).
Methods: Case-control study including 50 patients with CKD and 20 healthy volunteers.
Results: SAM level and SAM/SAH ratio in urine were significantly lower in patients than in control individuals (P <.
Capillary electrophoresis coupled with chloroform-acetonitrile extraction for rapid and highly selective determination of cysteine and homocysteine levels in human blood plasma and urine.
Electrophoresis
October 2017
A rapid and selective method has been developed for highly sensitive determination of total cysteine and homocysteine levels in human blood plasma and urine by capillary electrophoresis (CE) coupled with liquid-liquid extraction. Analytes were first derivatized with 1,1'-thiocarbonyldiimidazole and then samples were purified by chloroform-ACN extraction. Electrophoretic separation was performed using 0.
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