Stress-Related Gene Expression in Liver Tissues from Laying Hens Housed in Conventional Cage and Cage-Free Systems in the Tropics.

Global egg production is mainly based on cage systems, which have been associated with negative effects on the welfare of birds. Stress factors in restrictive production systems can lead to changes in gene transcription and protein synthesis, ultimately impacting the quality of poultry products. The liver serves various metabolic functions, such as glycogen storage, and plays a crucial role in animals' adaptation to environmental changes.

Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed.

Background And Aim: Heat shock proteins are highly conserved proteins that work as molecular chaperones expressed in response to thermal stress. This study aimed to determine the expression profile of genes related to the heat stress response in whole blood obtained from the Romosinuano creole breed.

Materials And Methods: Real-time polymerase chain reaction was performed to analyze the transcript of , , , and in the whole blood of Romosinuano under different temperature-humidity indices (THIs).

Identification of reference genes for expression studies in the liver and spleen of laying hens housed in cage and cage-free systems.

Background: The liver and spleen play a pivotal role in metabolism and immune response. During stress, neuroendocrine response induces changes in gene expression, and its assessment demands the validation of the stability of the reference genes to perform relative gene expression experiments.

Aim: The objective of this study was to determine the expression stability of four reference genes (, and ) in the liver and spleen tissues from laying hens housed in a conventional cage (CC) and cage-free (CF) egg production systems.

Molecular Detection of Virulence Factors in Isolated from Poultry and Human Samples.

Salmonellosis is a common infectious disease in humans caused by spp., which in recent years has shown an increase in its incidence, with products of avian origin being a common source of transmission. To present a successful infective cycle, there are molecular mechanisms such as virulence factors that provide characteristics that facilitate survival, colonization, and damage to the host.

Gene Expression of Aquaporins (AQPs) in Cumulus Oocytes Complex and Embryo of Cattle.

Aquaporins () are proteins with various functions related to proper cell function and early development in mammals. The aim of this study was to evaluate the presence of and determine their mRNA levels in the cumulus oocyte complex (COC) of four bovine breeds and in blastocysts of five bovine crosses. Grade I, II and III COCs were collected by ovum pick up from non-lactating heifers of the Brahaman, Holstein, Gir and Romosinuano breeds.

Molecular Characterization of spp. Isolates from Wild Colombian Babilla () Isolated In Situ.

enterica is a pathogen capable of colonizing various environments, including the intestinal tract of different animals such as mammals, birds, and reptiles, which can act as carriers. infection induces different clinical diseases, gastroenteritis being the most common, which in some cases, can evolve to septicemia and meningitis. Reptiles and amphibians have been reported as a reservoir of , and transmission of the pathogen to humans has been documented.

Molecular characterization of myelin basic protein a (mbpa) gene from red-bellied pacu (Piaractus brachypomus).

Background: Myelin basic protein (MBP) is one of the most important structural components of the myelin sheaths in both central and peripheral nervous systems. MBP has several functions including organization of the myelin membranes, reorganization of the cytoskeleton during the myelination process, and interaction with the SH3 domain in signaling pathways. Likewise, MBP has been proposed as a marker of demyelination in traumatic brain injury and chemical exposure.

Identification of Reference Genes for Expression Studies in the Whole-Blood from Three Cattle Breeds under Two States of Livestock Weather Safety.

Real-time PCR is widely used to study the relative abundance of mRNA due to its specificity, sensitivity, and repeatability quantification. However, relative quantification requires a reference gene, which should be stable in its expression, showing lower variation by experimental conditions or tissues. The aim of this study was to evaluate the stability of the expression of five commonly used reference genes , , , , and ) at different physiological stages (alert and emergency) in three different cattle breeds.

Molecular identification of fluoroquinolone resistance in spp. isolated from broiler farms and human samples obtained from two regions in Colombia.

Background And Aim: is one of the most common foodborne pathogens, the emergence of antibiotic-resistant strains of which is increasing. The aim of this study was to phenotypically and genotypically characterize the fluoroquinolone resistance of isolates from broiler and humans in two regions of Colombia.

Materials And Methods: strains (n49) were evaluated.

Heat stress on cattle embryo: gene regulation and adaptation.

Global warming has been affecting animal husbandry and farming production worldwide via changes in organisms and their habitats. In the tropics, these conditions are adverse for agriculture and animal production in some areas, due to high temperatures and relative humidity, affecting competitiveness related to economic activities. These environments have deteriorated livestock production, due to periods of drought, reduction in forage quality and heat stress, eliciting negative effects on reproduction, weight gain, and reduced meat and milk production.

Prevalence and Molecular Characterization of Isolated from Broiler Farms at the Tolima Region-Colombia.

is an important animal and human pathogen responsible for Salmonellosis, and it is frequently associated with the consumption of contaminated poultry products. The aim of this study was to estimate the prevalence of in the poultry farms and to determine the genetic relationship. A total of 135 samples collected from fifteen broiler farms, including cloacal, feed, water, environmental and farm operator faeces samples were subjected to microbiological isolation.

Antibiotic resistance in spp. isolated from poultry: A global overview.

is the most important foodborne pathogen, and it is often associated with the contamination of poultry products. Annually, causes around 93 million cases of gastroenteritis and 155,000 deaths worldwide. Antimicrobial therapy is the first choice of treatment for this bacterial infection; however, antimicrobial resistance has become a problem due to the misuse of antibiotics both in human medicine and animal production.

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for spp. isolated from poultry and humans.

Background And Aim: spp. are one of the most important food-borne pathogens in the world, emerging as a major public health concern. Moreover, multidrug-resistant (MDR) strains have been isolated from salmonellosis outbreaks, which compromise its treatment success.