Methacrylated chitosan/jellyfish collagen membranes as cell instructive platforms for liver tissue engineering.

Although the multidisciplinary area of liver tissue engineering is in continuous progress, research in this field is still focused on developing an ideal liver tissue template. Innovative strategies are required to improve membrane stability and bioactivity. In our study, sustainable biomimetic membranes were developed by blending methacrylated chitosan (CSMA) with jellyfish collagen (jCol) for liver tissue engineering applications.

Mild Approach for the Formulation of Chestnut Flour-Enriched Snacks: Influence of Processing Parameters on the Preservation of Bioactive Compounds of Raw Materials.

Third-generation snacks were developed from a triad of flours made up of chestnut, spelt, and chickpea flour. Optimal snack formulations and processing parameters have been established to ensure acceptable workability of the raw dough while protecting the bioactive components of the raw materials. The parameters examined were mixing time, speed, and temperature.

Effect of Cellulose-Based Bioplastics on Current LDPE Recycling.

The increased use of bioplastics in the market has led to their presence in municipal solid waste streams alongside traditional fossil-based polymers, particularly low-density polyethylene (LDPE), which bioplastics often end up mixed with. This study aimed to assess the impact of cellulose acetate plasticized with triacetin (CAT) on the mechanical recycling of LDPE. LDPE-CAT blends with varying CAT content (0%, 1%, 5%, 7.

Bioconversion of Cheese Whey and Food By-Products by into Fucoxanthin and n-3 Lc-PUFA through a Biorefinery Approach.

This study investigates the potential of utilizing three food wastes: cheese whey (CW), beet molasses (BM), and corn steep liquor (CSL) as alternative nutrient sources for the cultivation of the diatom , a promising source of polyunsaturated eicosapentaenoic acid (EPA) and the carotenoid fucoxanthin. The CW media tested did not significantly impact the growth rate of ; however, CW hydrolysate significantly enhances cell growth. BM in cultivation medium enhances biomass production and fucoxanthin yield.

The influence of bio-plastics for food packaging on combined anaerobic digestion and composting treatment of organic municipal waste.

The use of bio-plastic-based packaging as an alternative to conventional plastic packaging is increasing. Among the plethora of different bio-based plastics, the most relevant ones are those that, at the end of their life, can be treated with the organic fraction of municipal solid waste. Even in these cases, their impact on the waste processing and recycling is not always positive.

Use of Different Organic Carbon Sources in Cells: Effects on Biomass Productivity and Secondary Metabolites.

(Asteraceae family) is a perennial plant native to Mediterranean regions. This plant represents a source of high-value compounds, such as polyphenols and fatty acids that have several industrial applications. However, in vitro plant cell cultures can represent a valid alternative to in-field cultivation and facilitate the extraction of metabolites of commercial interest.

Formulation of New Media from Dairy and Brewery Wastes for a Sustainable Production of DHA-Rich Oil by .

Mozzarella stretching water (MSW) is a dairy effluent generated from mozzarella cheese production that does not have a real use and is destined to disposal, causing environmental problems and representing a high disposal cost for dairy producers. Spent brewery yeast (SBY) is another promising food waste produced after brewery manufacturing that could be recycled in new biotechnological processes. is an aquatic protist known as producer of bioactive lipids such as omega 3 long chain polyunsaturated fatty acids (ω3 LC-PUFA), in particular docosahexaenoic acid (DHA).

Sustainable production of food grade omega-3 oil using aquatic protists: Reliability and future horizons.

Biotechnological production of omega-3 polyunsaturated fatty acids (PUFAs) has become a commercial alternative to fish oil in the past twenty years. Compared to PUFA production by fatty fishes, that from microorganisms has increased due to its promising sustainability and high product safety and to increasing awareness in the expanding vegan market. Although autotrophic production by microalgae seems to be more sustainable in the long term, to date most of the microbial production of omega-3 is carried out under heterotrophic conditions using conventional fermentation technologies.

Polyethylene, Polystyrene, and Polypropylene leachate impact upon marine microalgae .

In the aquatic environment, plastics may release several hazardous substances of severe ecotoxicological concern not covalently bound to the polymers. The aim of this study was to examine the adverse effects of leachates of different virgin polymers, polypropylene (PP), polyethylene (PE), and polystyrene (PS) on marine microalgae . The tests carried out on included: growth inhibition, oxidative stress (DCFH-DA), and DNA damage (COMET assay).

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications.

Due to its unique properties, collagen is used in the growing fields of pharmaceutical and biomedical devices, as well as in the fields of nutraceuticals, cosmeceuticals, food and beverages. Collagen also represents a valid resource for bioplastics and biomaterials, to be used in the emerging health sectors. Recently, marine organisms have been considered as promising sources of collagen, because they do not harbor transmissible disease.

Adverse effects of oxo-degradable plastic leachates in freshwater environment.

The production of biodegradable plastics is considered to be a way to reduce plastic waste issue. Among others, oxo-degradant additives enable a faster degradation of plastics in the environment. However, the introduction of these new materials could provoke the release of substances potentially toxic in the environment.

Vegetable Tannin as a Sustainable UV Stabilizer for Polyurethane Foams.

A vegetable tannin, a flavonoid-type natural polyphenolic compound, was used to promote the stabilization of polyurethane foams against UV radiation. Several polyurethane foams were synthesized by using an isocyanate, and a mixture of ethoxylated cocoalkyl amine and vegetable tannin. The content of vegetable tannin was varied from 0 to 40 wt %.

Leachates of micronized plastic toys provoke embryotoxic effects upon sea urchin Paracentrotus lividus.

Microplastics are defined as plastic fragments <5 mm, and they are found in the ocean where they can impact on the ecosystem. Once released in seawater, microplastics can be internalized by organisms due to their small size, moreover they can also leach out several additives used in plastic manufacturing, such as plasticizers, flame retardants, etc., resulting toxic for biota.

Enhancing the EMI shielding of natural rubber-based supercritical CO foams by exploiting their porous morphology and CNT segregated networks.

Natural rubber/carbon nanotubes composite foams (F-NR/CNTs) with high electrical conductivity and excellent electromagnetic interference (EMI) performance were developed through a multi-step process including: (a) CNTs assembled on natural rubber latex particles, (b) pre-crosslinking of natural rubber, (c) supercritical carbon dioxide foaming of pre-crosslinked composite samples and (d) post-crosslinking of foamed composite samples. A closed-cell porous structure and a segregated CNT network are clearly observed in the resulting foams. Due to this morphology, F-NR/CNTs exhibit low density, good mechanical properties, and high electrical conductivity.

DNA damages and offspring quality in sea urchin Paracentrotus lividus sperms exposed to ZnO nanoparticles.

The recent advances in nanotechnology lead to a potential increase of the release of nanoparticles (NPs) into marine environment through different routes, with possible toxic effects upon the living part of this ecosystem. One of the ways of NPs marine contamination gaining today increasing concern stems from the widespread use cosmetics containing ZnO NPs as UV-filter. Although the possible adverse effects on marine organisms have been already ascertained, the information about the possible genotoxicity of ZnO NPs is still scant.

Early ecotoxic effects of ZnO nanoparticle chronic exposure in Mytilus galloprovincialis revealed by transcription of apoptosis and antioxidant-related genes.

Recently, China became one of the largest nanomaterial markets in the world. The wide use of ZnO nanoparticles in a number of products implies an increasing release in marine environment and consequently the evaluation of the potential effects upon marine organisms largely cultured in China for commercial purposes, such as invertebrate bivalves is a current need. To this aim, survival, bioaccumulation, and transcription pattern of key genes, p53, PDRP, SOD, CAT, and GST, involved in DNA damage/repair and antioxidation, in Mytilus galloprovincialis digestive gland, exposed to ZnO NPs (<100 nm) and ZnO bulk (150-200 nm) for 4 weeks, were evaluated.

Testing ZnO nanoparticle ecotoxicity: linking time variable exposure to effects on different marine model organisms.

Zinc oxide nanoparticles (ZnO NPs) are increasingly used in several personal care products, with high potential to be released directly into marine environment with consequent adverse impact on marine biota. This paper aimed to compare the ecotoxicological effect of ZnO NPs (< 100 nm) towards three marine organisms widely used in toxicity assessment: an algal species (Dunaliella tertiolecta), a bioluminescent bacterium (Vibrio fischeri), and a crustacean (Artemia salina). Bulk ZnO (ZnO bulk, 200 nm) and ionic zinc were also investigated for understanding the role of size and of ionic release in the ZnO toxic action.

Different sizes of ZnO diversely affected the cytogenesis of the sea urchin Paracentrotus lividus.

Today nanoparticles (NPs) have many applications in commercial products due to their small size and peculiar properties that, conversely, make them potentially toxic for humans and the environment. ZnO NPs are largely used in many personal care products, such as sunscreens and cosmetics. In this study the cytotoxic effects of ZnO particles with different sizes (ZnO Bulk, >100nm; ZnO NPs, 100nm and ZnO NPs, 14nm) upon the first developmental stages of the sea urchin Paracentrotus lividus, are evaluated.

Immune and reproductive system impairment in adult sea urchin exposed to nanosized ZnO via food.

In marine environment the release and the consequent sedimentation of ZnO NPs, mainly used in sunscreens, could provoke toxic effects in particular in grazer organisms, like sea urchins. In this work, a first evaluation of DNA and cellular effects on adult sea urchins Paracentrotus lividus exposed through the diet to different sizes (100 and 14nm) ZnO NPs, was performed. Moreover, the consequent impact upon offspring quality was evaluated.

Osteogenic differentiation of CD271(+) cells from rabbit bone marrow cultured on three phase PCL/TZ-HA bioactive scaffolds: comparative study with mesenchymal stem cells (MSCs).

Tissue engineering is one of the major challenges of orthopedics and trauma surgery for bone regeneration. Biomaterials filled with mesenchymal stem cells (MSCs) are considered the most promising approach in bone tissue engineering. Furthermore, our previous study showed that the multi-phase poly [ε-caprolactone]/thermoplastic zein-hydroxyapatite (PCL/TZ-HA) biomaterials improved rabbit (r) MSCs adhesion and osteoblast differentiation, thus demonstrating high potential of this bioengineered scaffold for bone regeneration.