Bougainvillea glabra Choisy bracts extract in free and liposomal forms reduce hyperplasia induced by let-60gain-of-function in Caenorhabditis elegans.

In this study, we evaluated the toxicological and antiproliferative effects of B. glabra Choisy bract extract (BGCE) in its free and loaded into liposomes forms administered to C. elegans mutants with let-60 gain-of-function (gf).

Non-esterified fatty acids and human lymphocyte death: a mechanism that involves calcium release and oxidative stress.

Although previous studies have shown that a mixture of fatty acids in similar proportion to that found in human plasma triggers apoptosis of peripheral blood lymphocytes from healthy subjects, the mechanism involved remains unknown. In the present study, we examined whether the effect of a mixture of fatty acids upon human lymphocyte death involves cytochrome c release from the mitochondria, activation of caspases 3, 6, 8, and 9, production of superoxide anion, nitric oxide (NO), increase in cytosolic Ca(2+) levels, and expression of the anti-apoptotic 14-3-3 and the pro-apoptotic FasL, bad, and bid proteins. Peripheral blood lymphocytes from healthy subjects were isolated and treated for up to 48 h with increasing concentrations (0.