Diagnostic Accuracy of Polymerase Chain Reaction Against Giemsa Staining on Tissue Biopsy for Cutaneous Leishmaniasis.

Objective: To evaluate the diagnostic accuracy of a commercial real-time polymerase chain reaction (PCR) kit targeting 18S rRNA against Giemsa-stained tissue slides in patients clinically suspected of cutaneous leishmaniasis (CL).

Study Design: Cross-sectional analytical study. Place and Duration of the Study: Department of Microbiology, Armed Forces Institute of Pathology / National University of Medical Sciences, Rawalpindi, Pakistan, from July to December 2022.

Frequency and Antibiogram of Isolates from Surgical Units of a Tertiary Care Hospital in Quetta.

Objective: To determine the frequency and antibiogram of the isolates from infected patients in surgical units of a tertiary care hospital.

Study Design: Cross-sectional, descriptive study.

Place And Duration Of Study: Department of Microbiology, Combined Military Hospital, Quetta, from March to October 2015.

Methicillin resistant coagulase negative staphylococcus: From colonizer to a pathogen.

The objective of our study was to determine the frequency of methicillin resistance in coagulase negative Staphylococcus (CoNS) and to determine its in-vitro antimicrobial susceptibility to various other routinely used antibiotics. It was a cross sectional study conducted at the department of Microbiology, Army Medical College, Rawalpindi, Pakistan from June 2011 to May 2012. The organisms were identified on the basis of colony morphology, Gram staining, catalase, DNAase and slide/tube coagulase tests.

IN VITRO SUSCEPTIBILITY PATTERN OF EXTENDED SPECTRUM β-LACTAMASE PRODUCING GRAM NEGATIVE BACILLI AGAINST TETRACYCLINES.

Background: Extended Spectrum β-lactamases (ESBLs) are emerging as common nosocomial pathogens and important cause of mortality and morbidity, if not treated properly. The need of the hour is to find effective treatment options for dealing with ESBL producing organisms. This study was aimed to evaluate in vitro susceptibility pattern of extended spectrum β-lactamase producers against tetracyclines.

In vitro Efficacy of Meropenem, Colistin and Tigecycline Against the Extended Spectrum Beta-Lactamase Producing Gram Negative Bacilli.

Objective: To compare the in vitro efficacy of meropenem, colistin and tigecycline against extended spectrum Betalactamase producing Gram negative bacilli by minimal inhibitory concentration.

Study Design: Cross-sectional descriptive study.

Place And Duration Of Study: Department of Microbiology, Army Medical College, National University of Sciences and Technology, Rawalpindi, from June to December 2010.

Frequency and antibiogram of vancomycin resistant enterococcus in a tertiary care hospital.

Objective: To determine the frequency of Vancomycin Resistant Enterococcus (VRE) in a tertiary care hospital of Rawalpindi, Pakistan.

Study Design: Observational, cross-sectional study.

Place And Duration Of Study: Department of Microbiology, Army Medical College, Rawalpindi, from May 2011 to May 2012.

Evaluation of different phenotypic methods for detection of amp C Beta-lactamase producing bacteria in clinical isolates.

Objective: To compare the sensitivity and specificity of different phenotypic methods for detection of Amp C betalactamase producing bacteria.

Study Design: Analytical study.

Place And Duration Of Study: Department of Microbiology, Army Medical College / National University of Sciences and Technology (NUST), Islamabad, Pakistan, from June 2010 to December 2010.

Frequency and antibiogram of multi-drug resistant Pseudomonas aeruginosa.

Objective: To find out the frequency and susceptibility pattern of multi-drug resistant (MDR) Pseudomonas aeruginosa in clinical specimens.

Study Design: Cross-sectional observational study.

Place And Duration Of Study: Department of Microbiology, Army Medical College, National University of Sciences and Technology (NUST), Rawalpindi, from January to September 2010.